Mouse Anti-HPRT1 Monoclonal Antibody (clone 1F8D11)
CAT#: VS7-0425-WR80
This product is a mouse IgG2a antibody that binds to human HPRT1. The antibody 1F8D11 can be used for WB and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6360/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/ihc_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/33265_A_7_8.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/33265_B_7_5.jpg
Normal Tissue
Figure 5 Bronchus
Respiratory epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/33265_B_6_2.jpg
Normal Tissue
Figure 6 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/33265_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12200/33265_A_9_4.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165704-HPRT1
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Specifications
- Immunogen
- Recombinant human HPRT1 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human HPRT1
- Species Reactivity
- Human
- Clone
- 1F8D11
- Molecule Class
- Transferases
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Lesch-Nyhan disease (LND); HPRT-related neurologic dysfunction (HND); HPRT-related hyperuricemia (HRH)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- HPRT; HGPRT
- Gene ID
- 3251
- UniProt ID
- P00492
- Long Name
- Hypoxanthine Phosphoribosyltransferase 1
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_000185.1
- Function
- Glycosyltransferase; Transferase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Clone 1F8D11"
- CAT
- Product Name
See other products for "HPRT1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3984z | Mouse Anti-HPRT1 Recombinant Antibody (clone 27D10) | WB, IP, ICC, IHC, ELISA | Mouse IgG2b, κ |
| MOB-1132MZ | Recombinant Mouse Anti-Human HPRT1 Antibody (clone 1F8D11) | FC | Mouse antibody |
| MRO-0767-CN | Rabbit Anti-HPRT1 Recombinant Antibody (clone JU03-26) | WB, IHC, IP | Rabbit IgG |
| VS3-XY817 | Mouse Anti-HPRT1 Recombinant Antibody (clone 1F8D11) | ELISA, WB | Mouse IgG2b |
| VS3-XY818 | Mouse Anti-HPRT1 Recombinant Antibody (clone 5F11A7) | ELISA, WB, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1670 | Rabbit Anti-HPRT1 Recombinant Antibody (clone DS1670AB) | WB, IP, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX541 | Mouse Anti-HPRT1 Recombinant Antibody (clone 571CT1.3.4) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1056 | Anti-HPRT1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3276 | Anti-Mouse HPRT1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3275 | Anti-Human HPRT1 Immunohistochemistry Kit | IHC |
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