Anti-HRAS Antibody Prodrug, Protease Activated (CBH03YJ)
CAT#: VS-1025-YC23
The Anti-HRAS Antibody Prodrug, Protease Activated (CBH03YJ) is an antibody prodrug comprised of a recombinant mouse antibody targeting human HRAS, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to HRAS protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to HRAS effectively. HRAS is a small GTPase and one of the three RAS family members that act as a central hub in numerous signaling pathways regulating cell growth. Activating point mutations in the HRAS gene are found in a variety of tumors, particularly those of the urinary tract and head and neck. These mutations lock the protein in its active state, driving continuous, oncogenic signaling.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70222/1357_B12_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2015/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49830/118215_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49830/118215_A_8_3.jpg
Normal Tissue
Figure 5 Gallbladder
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49830/118215_A_5_4.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49830/118215_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000174775-HRAS
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Specifications
- Host Animal
- Mouse
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human HRAS
- Target
- HRAS
- Species Reactivity
- Human
- Isotype
- IgG
- Clone
- CBH03YJ
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- HRAS belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. P21ras undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in HRAS cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, cognitive disability, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in HRAS are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma.
- Alternative Names
- CTLO; KRAS; HAMSV; HRAS1; KRAS2; RASH1; RASK2; Ki-Ras; p21ras; C-H-RAS; c-K-ras; H-RASIDX; c-Ki-ras; C-BAS/HAS; C-HA-RAS1; GTPase HRas; GTP- and GDP-binding peptide B
- Gene ID
- 3265
- UniProt ID
- P01112
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Related Diseases
Bladder Cancer
Downloadable Resources
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Datasheet
MSDS
COA
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Lot Number:
See other products for "Clone CBH03YJ"
- CAT
- Product Name
See other products for "HRAS"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1684z | Mouse Anti-HRAS Recombinant Antibody (clone 2H12) | WB, IF, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABZ-074 | Human Anti-HRAS Recombinant Antibody (clone YC106) | WB, IHC, IF, FuncS | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-074 | Human Anti-HRAS Recombinant Antibody (clone YC106); Fab Fragment | WB, IHC, IF, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-074 | Human Anti-HRAS Recombinant Antibody (clone YC106); scFv Fragment | WB, IHC, IF, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2323CT | Recombinant Mouse anti-Human HRAS Monoclonal antibody (UN60) | WB, ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0260MZ | Chicken Anti-HRAS Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-1076MZ | Chicken Anti-H-Ras Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-913CQ | Rat Anti-HRAS Recombinant Antibody (clone Y13-259) | WB, ICC, IF, IP, IHC-P, Neut, IHC-Fr | Rat IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-914CQ | Rat Anti-HRAS Recombinant Antibody (clone CBL947) | IF, IHC, IP, Neut, WB | Rat IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1674 | Rabbit Anti-HRAS Recombinant Antibody (clone DS1674AB) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0159-YJ | Mouse Anti-HRAS Recombinant Antibody (clone CBH03YJ) | ELISA, ICC, FuncS | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0159-YJ-S(P) | Mouse Anti-HRAS Recombinant Antibody (clone CBH03YJ); scFv Fragment | ELISA, ICC, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0159-YJ-F(E) | Mouse Anti-HRAS Recombinant Antibody (clone CBH03YJ); Fab Fragment | ELISA, ICC, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-1496CQ | Human Anti-HRAS Recombinant Antibody (clone Y13-238) | IP | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1294 | AbPlus™ Anti-HRAS Magnetic Beads (VS-0724-YC1294) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1058 | Anti-HRAS Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3280 | Anti-Mouse HRAS Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3279 | Anti-Human HRAS Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3281 | Anti-Canine HRAS Immunohistochemistry Kit | IHC |
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