Rabbit Anti-HSD3B1 Recombinant Antibody (VS13-YC551)
CAT#: VS13-YC551
The Rabbit Anti-HSD3B1 Recombinant Antibody is specific for human HSD3B1. HSD3B1 encodes an enzyme converting precursors to steroid hormones, involved in interconversion processes, important in hormone production pathways. The anti-HSD3B1 antibody can be used for Western blot (1/500-1/1000), Immunofluorescence (1/50-1/200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli, endoplasmic reticulum & cytokinetic bridge.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44028/575_B1_2_selected.jpg
Normal Tissue
Figure 2 Testis
Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43261/160469_A_6_6.jpg
Normal Tissue
Figure 3 Placenta
Trophoblastic cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43261/160469_A_2_7.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (CACO-2, SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000203857-HSD3B1
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Specifications
- Immunogen
- Recombinant human HSD3B1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HSD3B1
- Species Reactivity
- Human
- Applications
- WB, ICC, IF
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 10 mM PBS (pH 7.4) containing 150 mM NaCl, 50% Glycerol and 0.05% BSA.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunofluorescence (IF): 1/50-1/200
Target
- Alternative Names
- Hydroxy-Delta-5-Steroid Dehydrogenase, 3 Beta- And Steroid Delta-Isomerase 1; 3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type I; Short Chain Dehydrogenase/Reductase Family 11E, Member 1; Trophoblast Antigen FDO161G; 3-Beta-HSD I; HSDB3A; 3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 1; 3-Beta-Hydroxy-Delta(5)-Steroid Dehydrogenase; 3-Beta-Hydroxy-5-Ene Steroid Dehydrogenase; Delta-5-3-Ketosteroid Isomerase;
- Gene ID
- 3283
- UniProt ID
- P14060
- Long Name
- Hydroxy-Delta-5-Steroid Dehydrogenase, 3 Beta- And Steroid Delta-Isomerase 1
- Cellular Localization
- Endoplasmic reticulum membrane
- Function
- Signal transduction
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "HSD3B1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1141MZ | Recombinant Mouse Anti-Human HSD3B1 Antibody | FC, IHC-P, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0677MZ | Chicken Anti-3betaHSD Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1683 | Rabbit Anti-HSD3B1 Recombinant Antibody (clone DS1683AB) | WB, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY259 | Mouse Anti-NHP HSD3B1 Recombinant Antibody (clone FDO66Q) | IHC, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3292 | Anti-HSD3B1 Immunohistochemistry Kit | IHC |
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