CytoStream™ Rabbit Anti-HSF2 Recombinant Antibody (VS13-YC553)
CAT#: VS13-YC553
The CytoStream™ Rabbit Anti-HSF2 Recombinant Antibody is specific for human HSF2. HSF2 is transcription factor involved in heat shock response, activating genes under stress conditions with variants encoding distinct isoforms. The anti-HSF2 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31455/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells of seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31455/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human testis
Immunohistochemical staining of human testis shows distinct nuclear positivity in spermatogonia.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16067/ihc_selected.jpg
Normal Tissue
Figure 4 Testis
Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16067/40996_A_6_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Nuclear Non-germinal center cells
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16067/40996_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000025156-HSF2
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Specifications
- Immunogen
- Recombinant human HSF2.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HSF2
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 10 mM PBS (pH 7.4) containing 150 mM NaCl, 50% Glycerol and 0.05% BSA.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunofluorescence (IF): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "HSF2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1135MZ | Recombinant Mouse Anti-Human HSF2 Antibody (clone 832E2) | WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1686 | Rabbit Anti-HSF2 Recombinant Antibody (clone DS1686AB) | FC, ICC, IF, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY261 | Rat Anti-NHP HSF2 Recombinant Antibody (clone 3E2) | WB, IP | Rat IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3295 | Anti-HSF2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3296 | Anti-Mouse HSF2 Immunohistochemistry Kit | IHC |
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