Mouse Anti-HSP90AB1 Recombinant Antibody (ZG-0460J)
CAT#: ZG-0460J
This product is a mouse antibody that recognizes HSP90AB1.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-HSP90AB1 Recombinant Antibody (ZG-0460J) in WB
Western blot detection of Hsp90 beta in Hela,3T3,C6,CHO-K1 and COS7 cell lysates using Hsp90 beta mouse mAb (1:2000 diluted).Exposion time: 4min. Predicted band size:90KDa.Observed band size:90KDa.
ICC
Figure 2 Mouse Anti-HSP90AB1 Recombinant Antibody (ZG-0460J) in ICC
Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-Hsp90 beta mouse mAb (dilution 1:200).
IP
Figure 3 Mouse Anti-HSP90AB1 Recombinant Antibody (ZG-0460J) in IP
Immunoprecipitation analysis of Hela cell lysates using Hsp90 beta mouse mAb.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55729/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55729/958_C6_2_blue_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55729/911_A5_1_blue_red_green.jpg
Normal Tissue
Figure 4 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/ihc_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Goblet cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_8_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_8_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:MediumIntensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_6_6.jpg
Normal Tissue
Figure 10 Placenta
Decidual cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Trophoblastic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_2_7.jpg
Normal Tissue
Figure 11 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_8_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000096384-HSP90AB1
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Specifications
- Immunogen
- Purified recombinant human Hsp90 beta protein fragments expressed in
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HSP90AB1
- Species Reactivity
- Human, Mouse, Rat, Monkey, Hamster
- Applications
- WB, ICC
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunocytochemistry.
Target
- Alternative Names
- HSP84; HSPC2; HSPCB; D6S182; HSP90B; heat shock protein HSP 90-beta; HSP90-beta; heat shock 84 kDa; heat shock 90kD protein 1, beta; heat shock protein 90 kDa; heat shock protein 90kDa alpha (cytosolic), class B member 1; heat shock protein 90kDa alpha family class B member 1
- Gene ID
- 3326
- UniProt ID
- P08238
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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