Mouse Anti-HSP90AB1 Recombinant Antibody (ZG-0461J)
CAT#: ZG-0461J
This product is a mouse antibody that recognizes HSP90AB1.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-HSP90AB1 Recombinant Antibody (ZG-0461J) in WB
Western Blot analysis using HSP90β Monoclonal Antibody against Jurkat (1), A431 (2), HeLa (3), A549 (4), HEK293 (5), K562 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.
IHC
Figure 2 Mouse Anti-HSP90AB1 Recombinant Antibody (ZG-0461J) in IHC
Immunohistochemistry analysis of paraffin-embedded prostate cancer tissues (left) and lung cancer tissues (right) with DAB staining using HSP90 β Monoclonal Antibody.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55729/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55729/958_C6_2_blue_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55729/911_A5_1_blue_red_green.jpg
Normal Tissue
Figure 4 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/ihc_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Goblet cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_8_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_8_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:MediumIntensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_6_6.jpg
Normal Tissue
Figure 10 Placenta
Decidual cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Trophoblastic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_2_7.jpg
Normal Tissue
Figure 11 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5230/15840_A_8_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000096384-HSP90AB1
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Specifications
- Immunogen
- Purified recombinant fragment of human HSP90β expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HSP90AB1
- Species Reactivity
- Human, Mouse, Rat, Monkey
- Applications
- WB, IHC, ELISA
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunohistochemistry, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- HSP84; HSPC2; HSPCB; D6S182; HSP90B; heat shock protein HSP 90-beta; HSP90-beta; heat shock 84 kDa; heat shock 90kD protein 1, beta; heat shock protein 90 kDa; heat shock protein 90kDa alpha (cytosolic), class B member 1; heat shock protein 90kDa alpha family class B member 1
- Gene ID
- 3326
- UniProt ID
- P08238
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-516-F(E) | Recombinant Anti-human HSP90AB1 Antibody Fab Fragment | ELISA, RIA, FuncS | Fab |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-516-S(P) | Recombinant Anti-human HSP90AB1 Antibody scFv Fragment | WB, IHC, FuncS | scFv |
| MHH-516-S(P) | Recombinant Human Anti-human HSP90AB1 Antibody scFv Fragment | IF, FC, Biosensors, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1690 | Rabbit Anti-HSP90AB1 Recombinant Antibody (clone DS1690AB) | WB, IHC-P, ICC, IF, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC3385 | PE-A2/Human HSP90AB1 (ILDKKVEKV) MHC Tetramer | FCM | |
| MHC-LC3387 | PE-B*27:05/Human HSP90AB1 (RRVKEVVKK) MHC Tetramer | FCM | |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC555 | CytoStream™ Rabbit Anti-HSP90AB1 Recombinant Antibody (VS13-YC555) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
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