Rabbit Anti-HSP90B1 Recombinant Antibody (clone R02-9F8) (CAT#: VS3-XY3388)
The product is a recombinant rabbit antibody that recognizes HSP90B1 (Heat shock protein 90 beta family member 1), which participates in the unfolding of cytosolic leaderless cargos. It is capable of binding to human and mouse HSP90B1. It has been used in a variety of applications, such as Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3901/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3901/if_selected.jpg
Figure 2 IF staining of human cell line A431
(Immunofluorescent staining of human cell line A431 shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3901/14_F12_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A431 shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3901/14_F12_1_blue_red_green.jpg
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3901/16_F12_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3901/16_F12_1_blue_red_green.jpg
Figure 4 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23098_B_7_5.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23098_B_7_5.jpg
Figure 5 Thyroid gland
(Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23098_B_3_5.jpg
(Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23098_B_3_5.jpg
Figure 6 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23452_A_9_3.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23452_A_9_3.jpg
Figure 7 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23452_A_9_4.jpg
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23452_A_9_4.jpg
Figure 8 Kidney
(Bowman's capsule
Staining:High
Intensity: Strong
Quantity:>75%
Collecting ducts
Staining:High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:High
Intensity: Strong
Quantity:>75%
Proximal tubules (cell body)
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23452_A_7_5.jpg
(Bowman's capsule
Staining:High
Intensity: Strong
Quantity:>75%
Collecting ducts
Staining:High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:High
Intensity: Strong
Quantity:>75%
Proximal tubules (cell body)
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23452_A_7_5.jpg
Figure 9 Testis
(Pachytene spermatocytes
Staining:Medium
Intensity: Strong
Quantity: <25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23452_A_6_6.jpg
(Pachytene spermatocytes
Staining:Medium
Intensity: Strong
Quantity: <25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23452_A_6_6.jpg
Figure 10 Lymph node
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23452_A_9_8.jpg
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8424/23452_A_9_8.jpg
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000166598-HSP90B1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000166598-HSP90B1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse HSP90B1
- Species Reactivity
- Human, Mouse
- Clone
- R02-9F8
- Applications
- WB, IHC-P, ICC, IF
- Related Disease
- Pelizaeus-Merzbacher-Like Disease; Pseudoachondroplasia
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
- Gene ID
- 7184
- UniProt ID
- P14625
- Long Name
- Heat shock protein 90 beta family member 1
- Cellular Localization
- Endoplasmic reticulum lumen, Sarcoplasmic reticulum lumen, Melanosome
- Post Translation Modifications
- Glycosylation at Asn62, Asn107, Asn217, Asn445, Asn481, and Asn502 (NX_P14625 [NX_P14625-1])
Ubiquitination at Lys75, Lys142, Lys161, Lys168, Lys270, Lys434, and Lys671 (NX_P14625 [NX_P14625-1])
Modification sites at PhosphoSitePlus (P14625)
Glycosylation from GlyConnect ENPL_HUMAN (1206)
Glycosylation from GlyGen (P14625) 14 sites, 19 N-linked glycans (5 sites), 2 O-linked glycans (6 sites)
- Protein Refseq
- NP_003290.1
- Function
- Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity).
When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity).
Functions in endoplasmic reticulum associated degradation (ERAD) (PubMed:18264092).
Has ATPase activity.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1330-VHH | Recombinant Anti-Human HSP90B1 VHH Single Domain Antibody | FUNC, FA, IP, ChiP | Llama VHH |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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