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    Mouse Anti-HSPA9 Recombinant Antibody (clone 7E2E4) (CAT#: VS7-0425-WR97)

    This product is a mouse IgG2a recombinant antibody that binds to human HSPA9. The antibody 7E2E4 recognizes a specific amino acid domains of human HSPA9 (AA: 480-679). It can be used for WB, IHC, ICC, and FC assays. It cross reacts with mouse, monkey, and rat.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/if_selected.jpg
    Figure 2 IF staining of human cell line U-251 MG
    Figure 2 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/if_selected.jpg
    Figure 3 IHC staining of human cerebral cortex
    Figure 3 IHC staining of human cerebral cortex
    (Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/ihc_selected.jpg
    Figure 4 IHC staining of human kidney
    Figure 4 IHC staining of human kidney
    (Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/ihc_selected.jpg
    Figure 5 IF staining of human cell line A431
    Figure 5 IF staining of human cell line A431
    (Immunofluorescent staining of human cell line A431 shows localization to mitochondria.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/639_A10_1_blue_red_green.jpg
    Normal Tissue
    Figure 6 Cerebral cortex
    Figure 6 Cerebral cortex
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Glial cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Neuronal cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Neuropil
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_B_9_5.jpg
    Figure 7 Colon
    Figure 7 Colon
    (Glandular cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Peripheral nerve/ganglion
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_9_3.jpg
    Figure 8 Liver
    Figure 8 Liver
    (Cholangiocytes
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Hepatocytes
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_8_4.jpg
    Figure 9 Kidney
    Figure 9 Kidney
    (Cells in glomeruli
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Cells in tubules
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_9_5.jpg
    Figure 10 Testis
    Figure 10 Testis
    (Leydig cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Pachytene spermatocytes
    Staining:Medium
    Intensity: Strong
    Quantity: <25%
    Sertoli cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Spermatogonia cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_6_6.jpg
    Figure 11 Lymph node
    Figure 11 Lymph node
    (Germinal center cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Non-germinal center cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_7_8.jpg
    RNA Expression
    Figure 12 RNA cell line category: Low cell line specificity
    Figure 12 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000113013-HSPA9

    Specifications

    • Immunogen
    • Recombinant human HSPA9 (AA: 480-679) fragment expressed in E. Coli.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG2a
    • Specificity
    • Human HSPA9
    • Species Reactivity
    • Human, Mouse, Monkey, Rat
    • Clone
    • 7E2E4
    • Molecule Class
    • Heat Shock Protein
    • Applications
    • WB, IHC, ICC, FC
    • Conjugate
    • Unconjugated
    • Related Disease
    • Cancers; Neurodegenerative Disorders; Mitochondrial Diseases

    Product Property

    • Clonality
    • Monoclonal
    • Purity
    • >95% as determined by SDS-PAGE and SEC-HPLC analysis
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS
    • Preservative
    • 0.05% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • Western Blot: 1:500 - 1:2000
      Immunohistochemistry-Paraffin: 1:200 - 1:1000
      Immunocytochemistry: 1:200 - 1:1000
      Flow Cytometry: 1:200 - 1:400
      Enzyme-linked Immunosorbent Assay: 1:10000

    Target

    • Alternative Names
    • CSA; MOT; MOT2; SAAN; CRP40; EVPLS; GRP75; PBP74; GRP-75; HSPA9B; SIDBA4; MTHSP75; HEL-S-124m
    • Long Name
    • Heat Shock Protein Family A (Hsp70) Member 9
    • Cellular Localization
    • Cytoplasm; Mitochondrion; Nucleus
    • Post Translation Modifications
    • Acetylation; Methylation; Phosphoprotein
    • Function
    • May regulate the kinase DMPK.

    Recommended Products

    Isotype Control

    Secondary Antibody

    Reconstitution Buffer

    Recommended Dilution Buffer

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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    NAB-1346-sdAb Recombinant Anti-Human HSPA9 VHH Single Domain Antibody WB, IP, ICC, FC, FA Llama VHH

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