Mouse Anti-HSPA9 Recombinant Antibody (VS-0824-YJ46) (CAT#: VS-0824-YJ46)
52-3 is a mouse IgG recognizing the epitope located in aa 403-430. It doesn't have the capability of being internalized intracellularly. It has an affinity of KD = 8.17 nM to full length mortalin. 96-5 is a mouse monoclonal antibody binding to the epitope located in aa 310-410. It has the capability of being internalized intracellularly. It has an affinity of KD = 6.10 nM to full length mortalin. 38-4 is a mouse antibody that binds to the epitope aa 402-407, LFGRAP. It has the capability of being internalized intracellularly. It has an affinity of KD = 3.35 nM to full length mortalin.
| Sub Cat | Clone | Applications | Type | Size | Conjugation | Endotoxin | Purity | Fc Engineering | |
| VS-0824-YJ32 | 52-3 | ELISA | Mouse IgG2b kappa | ||||||
| VS-0824-YJ33 | 96-5 | ELISA, Internalization | Mouse IgG1 kappa | ||||||
| VS-0824-YJ34 | 38-4 | ELISA, Internalization | Mouse IgG1 kappa |
More Infomation
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/if_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/if_selected.jpg
Figure 3 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/ihc_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/ihc_selected.jpg
Figure 4 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/ihc_selected.jpg
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/ihc_selected.jpg
Figure 5 IF staining of human cell line A431
(Immunofluorescent staining of human cell line A431 shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/639_A10_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A431 shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/639_A10_1_blue_red_green.jpg
Figure 6 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_B_9_5.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_B_9_5.jpg
Figure 7 Colon
(Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_9_3.jpg
(Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_9_3.jpg
Figure 8 Liver
(Cholangiocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_8_4.jpg
(Cholangiocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_8_4.jpg
Figure 9 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_9_5.jpg
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_9_5.jpg
Figure 10 Testis
(Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Strong
Quantity: <25%
Sertoli cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_6_6.jpg
(Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Strong
Quantity: <25%
Sertoli cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_6_6.jpg
Figure 11 Lymph node
(Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_7_8.jpg
(Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_7_8.jpg
Figure 12 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000113013-HSPA9
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000113013-HSPA9
Specifications
- Immunogen
- Full-length mouse mortalin 2
- Host Species
- Mouse
- Specificity
- HSPA9
- Species Reactivity
- Human, Mouse
- Related Disease
- Congenital malformations and skeletal dysplasia
Product Property
- Purity
- >95% as determined by SDS-PAGE or SEC-HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- CSA; MOT; MOT2; SAAN; CRP40; EVPLS; GRP75; PBP74; GRP-75; HSPA9B; SIDBA4; MTHSP75; HEL-S-124m; stress-70 protein, mitochondrial; 75 kDa glucose-regulated protein; catecholamine-regulated protein 40; epididymis secretory sperm binding protein Li 124m; heat shock 70kD protein 9B; heat shock 70kDa protein 9 (mortalin); heat shock protein family A member 9; mortalin, perinuclear; mortalin-2; mortalin2; p66-mortalin; peptide-binding protein 74
- Gene ID
- 3313
- UniProt ID
- P15941
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1346-sdAb | Recombinant Anti-Human HSPA9 VHH Single Domain Antibody | WB, IP, ICC, FC, FA | Llama VHH |
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