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  • Mouse Anti-HSPA9 Recombinant Antibody (VS-0824-YJ46)

Mouse Anti-HSPA9 Recombinant Antibody (VS-0824-YJ46) (CAT#: VS-0824-YJ46)

52-3 is a mouse IgG recognizing the epitope located in aa 403-430. It doesn't have the capability of being internalized intracellularly. It has an affinity of KD = 8.17 nM to full length mortalin. 96-5 is a mouse monoclonal antibody binding to the epitope located in aa 310-410. It has the capability of being internalized intracellularly. It has an affinity of KD = 6.10 nM to full length mortalin. 38-4 is a mouse antibody that binds to the epitope aa 402-407, LFGRAP. It has the capability of being internalized intracellularly. It has an affinity of KD = 3.35 nM to full length mortalin.

Sub Cat Clone Applications Type Size Conjugation Endotoxin Purity Fc Engineering  
VS-0824-YJ32 52-3 ELISA Mouse IgG2b kappa
VS-0824-YJ33 96-5 ELISA, Internalization Mouse IgG1 kappa
VS-0824-YJ34 38-4 ELISA, Internalization Mouse IgG1 kappa
More Infomation
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/if_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/if_selected.jpg
Figure 3 IHC staining of human cerebral cortex
Figure 3 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/ihc_selected.jpg
Figure 4 IHC staining of human kidney
Figure 4 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/ihc_selected.jpg
Figure 5 IF staining of human cell line A431
Figure 5 IF staining of human cell line A431
(Immunofluorescent staining of human cell line A431 shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5219/639_A10_1_blue_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Figure 6 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_B_9_5.jpg
Figure 7 Colon
Figure 7 Colon
(Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_9_3.jpg
Figure 8 Liver
Figure 8 Liver
(Cholangiocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_8_4.jpg
Figure 9 Kidney
Figure 9 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_9_5.jpg
Figure 10 Testis
Figure 10 Testis
(Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Strong
Quantity: <25%
Sertoli cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_6_6.jpg
Figure 11 Lymph node
Figure 11 Lymph node
(Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/898/1961_A_7_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Figure 12 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000113013-HSPA9

Specifications

  • Immunogen
  • Full-length mouse mortalin 2
  • Host Species
  • Mouse
  • Specificity
  • HSPA9
  • Species Reactivity
  • Human, Mouse
  • Related Disease
  • Congenital malformations and skeletal dysplasia

Product Property

  • Purity
  • >95% as determined by SDS-PAGE or SEC-HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • CSA; MOT; MOT2; SAAN; CRP40; EVPLS; GRP75; PBP74; GRP-75; HSPA9B; SIDBA4; MTHSP75; HEL-S-124m; stress-70 protein, mitochondrial; 75 kDa glucose-regulated protein; catecholamine-regulated protein 40; epididymis secretory sperm binding protein Li 124m; heat shock 70kD protein 9B; heat shock 70kDa protein 9 (mortalin); heat shock protein family A member 9; mortalin, perinuclear; mortalin-2; mortalin2; p66-mortalin; peptide-binding protein 74

Recommended Products

Isotype Control

Secondary Antibody

Recommended Dilution Buffer

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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NAB-1346-sdAb Recombinant Anti-Human HSPA9 VHH Single Domain Antibody WB, IP, ICC, FC, FA Llama VHH

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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