Mouse Anti-HSPB1 Monoclonal Antibody (clone 5D7)
CAT#: VS7-0425-WR82
This product is a mouse IgG1 antibody that binds to human HSPB1. The antibody 5D7 can be used for WB, IHC, ICC, and FC assays. It cross reacts with rat.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47330/if_selected.jpg
Normal Tissue
Figure 2 Esophagus
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4439/17421_B_4_1_val_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: Low Intensity: Moderate Quantity: <25% Enterocytes -
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47331/104843_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47331/104843_A_7_5.jpg
Normal Tissue
Figure 5 High expression in vagina
Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47331/104843_B_2_2.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (hTCEpi, MCF7)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000106211-HSPB1
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Specifications
- Immunogen
- Recombinant human HSPB1 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human HSPB1
- Species Reactivity
- Human, Rat
- Clone
- 5D7
- Molecule Class
- Heat Shock Protein
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Alzheimer's disease
Parkinson's disease
Multiple sclerosis
Cancer
Cardiovascular disease
Stroke
Diabetes
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- CMT2F; HMN2B; HMND3; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
- Gene ID
- 3315
- UniProt ID
- P04792
- Long Name
- Heat Shock Protein Family B (Small) Member 1
- Cellular Localization
- Cytoplasm; Cytoskeleton; Nucleus
- Post Translation Modifications
- Acetylation; Methylation; Phosphoprotein
- Protein Refseq
- NP_001531.1
- Function
- Chaperone
REVIEWS AND Q&AS
CITATIONS
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "HSPB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1699 | Rabbit Anti-HSPB1 Recombinant Antibody (clone DS1699AB) | WB, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2094 | PE-B*07:02/Human HSPB1 (APAYSRAL) MHC Tetramer | FCM | |
| MHC-LC2095 | APC-B*07:02/Human HSPB1 (APAYSRAL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2337-CN | Rabbit Anti-HSPB1 Recombinant Antibody (clone CBACN-628) | WB, IF, IHC, IP | Rabbit IgG |
| MRO-2338-CN | Rabbit Anti-HSPB1 Recombinant Antibody (clone CBACN-629) | WB, IP | Rabbit IgG |
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| VS3-WK1361 | Mouse Anti-HSPB1 Antibody (clone Mix-mA) | WB, IHC-P | Mouse IgG |
| VS3-WK1433 | Mouse Anti-HSPB1 Recombinant Antibody (VS3-WK1433) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1360 | AbPlus™ Anti-HSPB1 Magnetic Beads (VS-0724-YC1360) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1076 | Anti-HSPB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3335 | Anti-Mouse HSPB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3334 | Anti-Human HSPB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3336 | Anti-Chicken HSPB1 Immunohistochemistry Kit | IHC |
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