Mouse Anti-HSPB1 Recombinant Antibody (clone SAIC-23C-35) (CAT#: FAMAB-1157CQ)

This product is a recombinant mouse antibody that recognizes HSPB1. The antibody was purified by affinity chromatography. It can be used in Immuno-MRM, WB.


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WB

Figure 1 Images of Western blots from screening the recombinant and hybridoma monoclonal antibodies (mAbs) using recombinant proteins, overexpressed lysates, and 6 breast cancer NCI60 cell line lysates.

Figure 1 Images of Western blots from screening the recombinant and hybridoma monoclonal antibodies (mAbs) using recombinant proteins, overexpressed lysates, and 6 breast cancer NCI60 cell line lysates.

The purified mAbs were run as controls alongside the recombinant proteins or the overexpressed lysate-proteins. Because the mAbs' protein targets (except for TSHB and CGB) were expected to be detected in breast cancer cell lines. For the Western blots using the NCI60 breast cancer cell lines.

Schoenherr, R. M., Saul, R. G., Whiteaker, J. R., Yan, P., Whiteley, G. R., & Paulovich, A. G. (2015). Anti-Peptide Monoclonal Antibodies Generated for Immuno-Multiple Reaction Monitoring-Mass Spectrometry Assays Have a High Probability of Supporting Western blot and ELISA*[S]. Molecular & cellular proteomics, 14(2), 382-398.


Specifications

  • Immunogen
  • Peptide VSLDVNHFAPDELTVK conjugated to KLH.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Human HSPB1
  • Species Reactivity
  • Human
  • Clone
  • SAIC-23C-35
  • Applications
  • Immuno-MRM, WB

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • Heat Shock Protein Family B (Small) Member 1; Estrogen-Regulated 24 KDa Protein; Stress-Responsive Protein 27; Heat Shock 27kDa Protein 1; Heat Shock 27kD Protein 1; Heat Shock 27 KDa Protein; 28 KDa Heat Shock Protein; HSP27; HSP28; SRP27

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1699 Rabbit Anti-HSPB1 Recombinant Antibody (clone DS1699AB) WB, IHC, IP, FC Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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