Mouse Anti-HSPB1 Recombinant Antibody (VS3-CJ377) (CAT#: VS3-CJ377)

• Immunogen
• Recombinant protein corresponding to human HSP27

• Host Species
• Mouse

• Specificity
• Human HSPB1

• Species Reactivity
• Human

• Applications
• WB, IHC

• Conjugate
• Unconjugated

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 30% Glycerol, 0.87% NaCl, 0.42% Potassium phosphate, pH7.3.

• Preservative
• 0.01% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Western Blot (1:1000-1:2000), Immunohistochemistry (1:50-1:200).

• Alternative Names
• CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102

• Gene ID
• 3315

• UniProt ID
• P04792

• Sequence Similarities
• Belongs to the small heat shock protein (HSP20) family.

• Cellular Localization
• Cytoplasm, Cytoskeleton, Nucleus

• Post Translation Modifications
• Phosphorylated upon exposure to protein kinase C activators and heat shock (PubMed:8325890).
  Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement (PubMed:1332886, PubMed:8093612, PubMed:19166925).

• Protein Refseq
• NP_001531.1

• Function
• Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975).
  Plays a role in stress resistance and actin organization (PubMed:19166925).
  Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).

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