Mouse Anti-HSPB1 Recombinant Antibody (ZG-0450J)
CAT#: ZG-0450J
This product is a mouse antibody that recognizes HSPB1.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-HSPB1 Recombinant Antibody (ZG-0450J) in WB
Western blot detection of Hsp27 in Hela,HsHela,A431,COS7,Lncap and MCF7 cell lysates and using Hsp27 mouse mAb (1:1000 diluted). Predicted band size: 23KDa.Observed band size:27KDa.
ICC
Figure 2 Mouse Anti-HSPB1 Recombinant Antibody (ZG-0450J) in ICC
mmunocytochemistry stain of Hela using Hsp27 mouse mAb (1:300).
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47330/if_selected.jpg
Normal Tissue
Figure 2 Esophagus
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4439/17421_B_4_1_val_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: Low Intensity: Moderate Quantity: <25% Enterocytes -
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47331/104843_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47331/104843_A_7_5.jpg
Normal Tissue
Figure 5 High expression in vagina
Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47331/104843_B_2_2.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (hTCEpi, MCF7)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000106211-HSPB1
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Specifications
- Immunogen
- Purified recombinant human Hsp27 protein fragments expressed in E.coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HSPB1
- Species Reactivity
- Human, Monkey
- Applications
- WB, ICC
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunocytochemistry.
Target
- Alternative Names
- CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102; heat shock protein beta-1; 28 kDa heat shock protein; epididymis secretory protein Li 102; estrogen-regulated 24 kDa protein; heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; putative heat shock protein family B member 1 isoform; stress-responsive protein 27
- Gene ID
- 3315
- UniProt ID
- P04792
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "HSPB1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1699 | Rabbit Anti-HSPB1 Recombinant Antibody (clone DS1699AB) | WB, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2094 | PE-B*07:02/Human HSPB1 (APAYSRAL) MHC Tetramer | FCM | |
| MHC-LC2095 | APC-B*07:02/Human HSPB1 (APAYSRAL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1942-CN | Rabbit Anti-HSPB1 Polyclonal Antibody (MRO-1942-CN) | WB, IF, IHC | Rabbit IgG |
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| ZG-0753J | Rabbit Anti-HSPB1 Recombinant Antibody (clone 10C11) | ELISA, WB, IHC, IF, FC, IP | Rabbit IgG |
| VS3-FY711 | Recombinant Rabbit Anti-HSPB1 Antibody (clone R03-9F4) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1360 | AbPlus™ Anti-HSPB1 Magnetic Beads (VS-0724-YC1360) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1076 | Anti-HSPB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3335 | Anti-Mouse HSPB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3334 | Anti-Human HSPB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3336 | Anti-Chicken HSPB1 Immunohistochemistry Kit | IHC |
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