AbPlus™ Anti-HSPD1 Magnetic Beads (VS-0724-YC1197)
CAT#: VS-0724-YC1197
The AbPlus Anti-HSPD1 Magnetic Beads (VS-0724-YC1197) is an innovative affinity resin which is bound with anti-HSPD1 specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of HSPD1 protein under native and denaturing conditions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/50025/if_selected.jpg
Subcellular Location
Figure 3 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of renal tubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/ihc_selected.jpg
Subcellular Location
Figure 4 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity, with a granular pattern, in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2775/ihc_selected.jpg
Subcellular Location
Figure 5 IHC staining of human colon
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72816/ihc_selected.jpg
Subcellular Location
Figure 6 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/29_C6_1_blue_red_green.jpg
Subcellular Location
Figure 7 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_B_8_5.jpg
Normal Tissue
Figure 8 Colon
Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_7_3.jpg
Normal Tissue
Figure 9 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_9_4.jpg
Normal Tissue
Figure 10 Pancreas
Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_1_3.jpg
Normal Tissue
Figure 11 Kidney
Bowman's capsule
Staining: High
Intensity: Strong
Quantity:>75%
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining: High
Intensity: Strong
Quantity:>75%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_8_5.jpg
Normal Tissue
Figure 12 Testis
Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_6_6.jpg
RNA Expression
Figure 13 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000144381-HSPD1
❮
❯
Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-HSPD1 specific antibody
- Target
- HSPD1
- Immunogen
- Synthetic peptide corresponding to the C-terminus of the human HSPD1/HSP60.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]
- Alternative Names
- Heat Shock Protein Family D (Hsp60) Member 1; Heat Shock 60kDa Protein 1 (Chaperonin); Mitochondrial Matrix Protein P1; P60 Lymphocyte Protein; 0 KDa Chaperonin; Chaperonin 60; HuCHA60; HSP-60; HSP60; CPN60; Spastic Paraplegia 13 (Autosomal Dominant);
- Gene ID
- 3329
- UniProt ID
- P10809
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-920CQ | Mouse Anti-HSPD1 Recombinant Antibody (clone 4B9/89) | Block, ELISA, ICC, IF, IHC, IHC-P, IP, WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
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| MOR-1704 | Rabbit Anti-HSPD1 Recombinant Antibody (clone DS1704AB) | IHC-P, WB | Rabbit IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2077 | PE-A*02:01/Human HSPD1 (KLSDGVAVL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-1428LC | Mouse Anti-HSPD1 Recombinant Antibody (clone 9C9) | ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0119JF-S(P) | Mouse Anti-HSPD1 Recombinant Antibody (clone II-13); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
| FAMAB-0120JF-S(P) | Mouse Anti-HSPD1 Recombinant Antibody (clone ML-30); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ576 | Mouse Anti-HSPD1 Recombinant Antibody (VS3-CJ576) | ELISA, WB | Mouse IgG1 |
| VS3-CJ577 | Mouse Anti-HSPD1 Recombinant Antibody (clone PT1700) | WB, IF, IHC-P | Mouse IgG1, κ |
| VS3-CJ578 | Mouse Anti-HSPD1 Recombinant Antibody (clone ABT-HSP60) | IF, IHC-P | Mouse IgG1, κ |
| VS3-FY712 | Recombinant Rabbit Anti-HSPD1 Antibody (clone R02-5B7) | WB, IHC-P | Rabbit IgG |
| VS3-FY713 | Recombinant Rabbit Anti-HSPD1 Antibody (clone R02-8D9) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY134 | AbPlus™ Anti-HSPD1 Magnetic Beads (VS-0424-XY134) | IP, Protein Purification | |
| VS-0724-YC1195 | AbPlus™ Anti-Hspd1 Magnetic Beads (VS-0724-YC1195) | IP, Protein Purification |
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