Rabbit Anti-HSPD1 Recombinant Antibody (clone 3D8)
CAT#: ZG-0754J
This product is a rabbit antibody that recognizes HSPD1.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-HSPD1 Antibody (ZG-0754J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, Mouse brain tissue
All lanes: HSPD1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 62, 18 kDa
Observed band size: 60 kDa
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/if_selected.jpg
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Specifications
- Immunogen
- A synthesized peptide derived from human Hsp60
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HSPD1
- Species Reactivity
- Human, Mouse
- Clone
- 3D8
- Applications
- ELISA, WB, IHC, IP
- Conjugate
- Uconjugated
- Related Disease
- Autosomal recessive spastic paraplegia
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunoprecipitation.
WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000
Target
- Alternative Names
- HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60; 60 kDa heat shock protein, mitochondrial; 60 kDa chaperonin; P60 lymphocyte protein; chaperonin 60; epididymis secretory sperm binding protein; heat shock 60kDa protein 1 (chaperonin); heat shock protein 65; mitochondrial matrix protein P1; short heat shock protein 60 Hsp60s1
- Gene ID
- 3329
- UniProt ID
- P10809
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| MOR-1704 | Rabbit Anti-HSPD1 Recombinant Antibody (clone DS1704AB) | IHC-P, WB | Rabbit IgG |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-1428LC | Mouse Anti-HSPD1 Recombinant Antibody (clone 9C9) | ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0775-CN | Mouse Anti-HSPD1 Recombinant Antibody (clone 9-49) | WB, IF, IHC, FC | Mouse IgG2a |
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| VS3-FY713 | Recombinant Rabbit Anti-HSPD1 Antibody (clone R02-8D9) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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