Rabbit Anti-HSPD1 Recombinant Antibody (clone 3D8) (CAT#: ZG-0754J)
This product is a rabbit antibody that recognizes HSPD1.
Specific Inquiry
WB
Figure 1 Rabbit Anti-HSPD1 Antibody (ZG-0754J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, Mouse brain tissue
All lanes: HSPD1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 62, 18 kDa
Observed band size: 60 kDa
IHC
Figure 2 Rabbit Anti-HSPD1 Antibody (ZG-0754J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC
Figure 3 Rabbit Anti-HSPD1 Antibody (ZG-0754J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IP
Figure 4 Rabbit Anti-HSPD1 Antibody (ZG-0754J) in IP
Immunoprecipitating HSPD1 in HepG2 whole cell lysate
Lane 1: Rabbit control IgG in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0754J (3µg)+ HepG2 whole cell lysate (500µg)
Lane 3: HepG2 whole cell lysate (10µg)
Specifications
- Immunogen
- A synthesized peptide derived from human Hsp60
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HSPD1
- Species Reactivity
- Human, Mouse
- Clone
- 3D8
- Applications
- ELISA, WB, IHC, IP
- Conjugate
- Uconjugated
- Related Disease
- Autosomal recessive spastic paraplegia
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunoprecipitation.
WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000
Target
- Alternative Names
- HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60; 60 kDa heat shock protein, mitochondrial; 60 kDa chaperonin; P60 lymphocyte protein; chaperonin 60; epididymis secretory sperm binding protein; heat shock 60kDa protein 1 (chaperonin); heat shock protein 65; mitochondrial matrix protein P1; short heat shock protein 60 Hsp60s1
- Gene ID
- 3329
- UniProt ID
- P10809
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1035 | Mouse Anti-HSPD1 Recombinant Antibody (clone YC181); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0119JF-F(E) | Mouse Anti-HSPD1 Recombinant Antibody (clone II-13); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
| FAMAB-0120JF-F(E) | Mouse Anti-HSPD1 Recombinant Antibody (clone ML-30); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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