Mouse Anti-HSPD1 Recombinant Antibody (clone ML-30)
CAT#: FAMAB-0120JF
Recombinant monoclonal antibody specially binds to HSP60. This mouse anti-HSP60 antibody ML-30 could be wildly used in the treatment of atherosclerosis.
Published Data
Gene Expression
IHC
Figure 1 Immunohistochemical analysis of HSPD1 abundance in kidney specimens from mice.
Normal indicates the normal control group.
Fang, Y., Xie, T., Xue, N., Kuang, Q., Wei, Z., Liang, M., & Ding, X. (2017). miR-382 contributes to renal tubulointerstitial fibrosis by downregulating HSPD1. Oxidative medicine and cellular longevity, 2017.
WB
Figure 2 Protein expression of Bax, HSPD1, and Trx was normalized to GAPDH in the obstructed kidneys treated with anti-miR and siRNA from the experiment above.
Direct siRNA-mediated suppression of HSPD1 in the UUO kidney promoted oxidative stress despite miR-382 blockade.
Fang, Y., Xie, T., Xue, N., Kuang, Q., Wei, Z., Liang, M., & Ding, X. (2017). miR-382 contributes to renal tubulointerstitial fibrosis by downregulating HSPD1. Oxidative medicine and cellular longevity, 2017.
IF
Figure 3 Representative images of kidney tissue with Sirius red staining and 3-NT immunofluorescence from animals undergoing UUO surgery and receiving a combination of HSPD1 or control siRNA.
3-NT (green) and DAPI (blue) immunofluorescence from control and anti-miR382/HSPD1 siRNA-treated mice are displayed.
Fang, Y., Xie, T., Xue, N., Kuang, Q., Wei, Z., Liang, M., & Ding, X. (2017). miR-382 contributes to renal tubulointerstitial fibrosis by downregulating HSPD1. Oxidative medicine and cellular longevity, 2017.
ELISA
Figure 4 ELISA assay of 3-NT (Abcam) with the cell homogenate.
Statistically significant (P< 0.01) when compared with the TGF-β1 group.
Fang, Y., Xie, T., Xue, N., Kuang, Q., Wei, Z., Liang, M., & Ding, X. (2017). miR-382 contributes to renal tubulointerstitial fibrosis by downregulating HSPD1. Oxidative medicine and cellular longevity, 2017.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/50025/if_selected.jpg
Subcellular Location
Figure 3 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of renal tubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/ihc_selected.jpg
Subcellular Location
Figure 4 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity, with a granular pattern, in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2775/ihc_selected.jpg
Subcellular Location
Figure 5 IHC staining of human colon
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72816/ihc_selected.jpg
Subcellular Location
Figure 6 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/29_C6_1_blue_red_green.jpg
Subcellular Location
Figure 7 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_B_8_5.jpg
Normal Tissue
Figure 8 Colon
Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_7_3.jpg
Normal Tissue
Figure 9 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_9_4.jpg
Normal Tissue
Figure 10 Pancreas
Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_1_3.jpg
Normal Tissue
Figure 11 Kidney
Bowman's capsule
Staining: High
Intensity: Strong
Quantity:>75%
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining: High
Intensity: Strong
Quantity:>75%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_8_5.jpg
Normal Tissue
Figure 12 Testis
Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_6_6.jpg
RNA Expression
Figure 13 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000144381-HSPD1
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human HSP60
- Species Reactivity
- Human
- Clone
- ML-30
- Applications
- WB, ELISA, IHC, IF
- Related Disease
- Atherosclerosis
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HSPD1 antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Immunofluorescence.
Target
- Alternative Names
- HSPD1; heat shock 60kDa protein 1 (chaperonin); HLD4; CPN60; GROEL; HSP60; HSP65; SPG13
- Gene ID
- 3329
- UniProt ID
- A0A024R3X4
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1035 | Mouse Anti-HSPD1 Recombinant Antibody (clone YC181); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0119JF-F(E) | Mouse Anti-HSPD1 Recombinant Antibody (clone II-13); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
| FAMAB-0120JF-F(E) | Mouse Anti-HSPD1 Recombinant Antibody (clone ML-30); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-110CT | Anti-Human HSP60 Recombinant Antibody (prozumab) | Inhibit, ELISA | Humanized antibody |
| TAB-110CT-S(P) | Anti-Human HSP60 Recombinant Antibody scFv Fragment (prozumab) | Inhibit, ELISA | Humanized antibody |
| TAB-110CT-F(E) | Anti-Human HSP60 Recombinant Antibody Fab Fragment (prozumab) | Inhibit, ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-018CT | Recombinant Mouse Anti-HSP60 Antibody | WB, IHC, FC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0880CT | Recombinant Mouse anti-Human HSPD1 Monoclonal antibody (2E22CE9) | FC, ICC, IF, ELISA, WB | |
| MRO-1944-CN | Rabbit Anti-HSPD1 Polyclonal Antibody (MRO-1944-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1945-CN | Rabbit Anti-HSPD1 Polyclonal Antibody (MRO-1945-CN) | WB, IF, IHC | Rabbit IgG |
| ZG-0754J | Rabbit Anti-HSPD1 Recombinant Antibody (clone 3D8) | ELISA, WB, IHC, IP | Rabbit IgG |
| VS3-FY2741 | Rabbit Anti-HSPD1 Recombinant Antibody (VS3-FY2741) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-920CQ | Mouse Anti-HSPD1 Recombinant Antibody (clone 4B9/89) | Block, ELISA, ICC, IF, IHC, IHC-P, IP, WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1704 | Rabbit Anti-HSPD1 Recombinant Antibody (clone DS1704AB) | IHC-P, WB | Rabbit IgG |
| MOR-0077-FY | Rabbit Anti-HSPD1 Recombinant Antibody (clone AFY0048) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2077 | PE-A*02:01/Human HSPD1 (KLSDGVAVL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-1428LC | Mouse Anti-HSPD1 Recombinant Antibody (clone 9C9) | ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF309 | Qa-1b/Human Hsp60 (GMKFDRGYI) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0119JF-S(P) | Mouse Anti-HSPD1 Recombinant Antibody (clone II-13); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
| FAMAB-0120JF-S(P) | Mouse Anti-HSPD1 Recombinant Antibody (clone ML-30); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY134 | AbPlus™ Anti-HSPD1 Magnetic Beads (VS-0424-XY134) | IP, Protein Purification | |
| VS-0724-YC1195 | AbPlus™ Anti-Hspd1 Magnetic Beads (VS-0724-YC1195) | IP, Protein Purification | |
| VS-0724-YC1197 | AbPlus™ Anti-HSPD1 Magnetic Beads (VS-0724-YC1197) | IP, Protein Purification |
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