Mouse Anti-HSPD1 Recombinant Antibody (clone ML-30) (CAT#: FAMAB-0120JF)
Recombinant monoclonal antibody specially binds to HSP60. This mouse anti-HSP60 antibody ML-30 could be wildly used in the treatment of atherosclerosis.
Specific Inquiry
IHC
Figure 1 Immunohistochemical analysis of HSPD1 abundance in kidney specimens from mice.
Normal indicates the normal control group.
Fang, Y., Xie, T., Xue, N., Kuang, Q., Wei, Z., Liang, M., & Ding, X. (2017). miR-382 contributes to renal tubulointerstitial fibrosis by downregulating HSPD1. Oxidative medicine and cellular longevity, 2017.
WB
Figure 2 Protein expression of Bax, HSPD1, and Trx was normalized to GAPDH in the obstructed kidneys treated with anti-miR and siRNA from the experiment above.
Direct siRNA-mediated suppression of HSPD1 in the UUO kidney promoted oxidative stress despite miR-382 blockade.
Fang, Y., Xie, T., Xue, N., Kuang, Q., Wei, Z., Liang, M., & Ding, X. (2017). miR-382 contributes to renal tubulointerstitial fibrosis by downregulating HSPD1. Oxidative medicine and cellular longevity, 2017.
IF
Figure 3 Representative images of kidney tissue with Sirius red staining and 3-NT immunofluorescence from animals undergoing UUO surgery and receiving a combination of HSPD1 or control siRNA.
3-NT (green) and DAPI (blue) immunofluorescence from control and anti-miR382/HSPD1 siRNA-treated mice are displayed.
Fang, Y., Xie, T., Xue, N., Kuang, Q., Wei, Z., Liang, M., & Ding, X. (2017). miR-382 contributes to renal tubulointerstitial fibrosis by downregulating HSPD1. Oxidative medicine and cellular longevity, 2017.
ELISA
Figure 4 ELISA assay of 3-NT (Abcam) with the cell homogenate.
Statistically significant (P< 0.01) when compared with the TGF-β1 group.
Fang, Y., Xie, T., Xue, N., Kuang, Q., Wei, Z., Liang, M., & Ding, X. (2017). miR-382 contributes to renal tubulointerstitial fibrosis by downregulating HSPD1. Oxidative medicine and cellular longevity, 2017.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human HSP60
- Species Reactivity
- Human
- Clone
- ML-30
- Applications
- WB, ELISA, IHC, IF
- Related Disease
- Atherosclerosis
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HSPD1 antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Immunofluorescence.
Target
- Alternative Names
- HSPD1; heat shock 60kDa protein 1 (chaperonin); HLD4; CPN60; GROEL; HSP60; HSP65; SPG13
- Gene ID
- 3329
- UniProt ID
- A0A024R3X4
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1035 | Mouse Anti-HSPD1 Recombinant Antibody (clone YC181); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0119JF-F(E) | Mouse Anti-HSPD1 Recombinant Antibody (clone II-13); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
| FAMAB-0120JF-F(E) | Mouse Anti-HSPD1 Recombinant Antibody (clone ML-30); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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