Human Anti-HTRA1 Recombinant Antibody (clone Galegenimab)
CAT#: VS-0723-WK18
This product is a humanized IgG1 antibody that recognizes human HTRA1, which is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Galegenimab is recommended for flow cytometry at a dilution of 1:100.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line LHCN-M2
Immunofluorescent staining of human cell line LHCN-M2 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36655/1781_F1_29_cr596dc90203c13_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36655/158641_A_8_2_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36655/421_D4_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36655/427_D4_1_blue_red_green.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36655/158641_A_8_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36655/158641_A_7_5.jpg
Normal Tissue
Figure 7 Ovary
Follicle cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36655/158641_A_5_7.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, fHDF/TERT166, HSkMC, LHCN-M2, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000166033-HTRA1
❮
❯
SDS-PAGE
Figure 1 Anti-HTRA1 Recombinant Antibody (VS-0723-WK18) in SDS-PAGE
SDS-PAGE analysis of VS-0723-WK18 in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 2 Anti-HTRA1 Recombinant Antibody (VS-0723-WK18) in SEC-HPLC
SEC-HPLC analysis of VS-0723-WK18 shows that the purity is more than 95%.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µlInjection: 10 µl
WB
Figure 3 Anti-HTRA1 Recombinant Antibody (VS-0723-WK18) in WB
Western blot analysis of VS-0723-WK18 was performed by loading human HTRA1 protein (Lane 1 0.1 μg, Lane 2 0.3 μg, Lane 3 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a NC membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with VS-0723-WK18 and HRP goat anti-human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
DB
Figure 4 Anti-HTRA1 Recombinant Antibody (VS-0723-WK18) in DB
Dot Blot analysis of VS-0723-WK18 was performed by coating with human HTRA1 protein (His tag).
VS-0723-WK18 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000)
ELISA
Figure 5 Anti-HTRA1 Recombinant Antibody (VS-0723-WK18) in ELISA
ELISA analysis of VS-0723-WK18 was performed by coating with human HTRA1 protein (His tag). Then blocked with BSA and incubated with VS-0723-WK18. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
❮
❯
Specifications
- Immunogen
- Recombinant human HTRA1 protein
- Host Species
- Human
- Derivation
- Humanized
- Type
- Human IgG1
- Specificity
- Human HTRA1
- Species Reactivity
- Human
- Clone
- Galegenimab
- Applications
- FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95%
- Format
- Lyophilized
- Buffer
- Lyophilized from sterile PBS (pH 7.4) with 5%-8% trehalose.
- Preservative
- No Preservative
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Flow Cytometry: 1/100
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0723-WK18. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "HTRA1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1362-sdAb | Recombinant Anti-Human HTRA1 VHH Single Domain Antibody | IP, ChiP, IHC, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-113CT | Human Anti-HtrA1 Recombinant Antibody (TAB-113CT) | ELISA, WB | Human IgG1 |
| TAB-113CT-S(P) | Human Anti-HtrA1 Recombinant Antibody; scFv Fragment (TAB-113CT-S(P)) | ELISA, WB | Human scFv |
| TAB-113CT-F(E) | Human Anti-HtrA1 Recombinant Antibody; Fab Fragment (TAB-113CT-F(E)) | ELISA, WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0526-CN | Mouse Anti-HTRA1 Recombinant Antibody (HPAB-0526-CN) | FuncS, WB, Inhib | Mouse IgG2a |
| HPAB-0527-CN | Mouse Anti-HTRA1 Recombinant Antibody (HPAB-0527-CN) | IHC, WB, Inhib | Mouse IgG2a |
| HPAB-0529-CN | Human Anti-HTRA1 Recombinant Antibody (HPAB-0529-CN) | WB, ELISA, FC, IHC | Humanized IgG |
| MOB-092CQ | Mouse Anti-HTRA1 Recombinant Antibody (clone YC109) | ELISA, WB, IF, IP | Mouse IgG1, κ |
| VS3-FY2191 | Rabbit Anti-HTRA1 Recombinant Antibody (clone R22-3C-3) | WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0526-CN-F(E) | Mouse Anti-HTRA1 Recombinant Antibody; Fab Fragment (HPAB-0526-CN-F(E)) | WB, ELISA, FC, IHC | Mouse Fab |
| HPAB-0527-CN-F(E) | Mouse Anti-HTRA1 Recombinant Antibody; Fab Fragment (HPAB-0527-CN-F(E)) | WB, ELISA, FC, IHC | Mouse Fab |
| HPAB-0529-CN-F(E) | Human Anti-HTRA1 Recombinant Antibody; Fab Fragment (HPAB-0529-CN-F(E)) | WB, ELISA, FC, IHC | Humanized Fab |
| HPAB-0530-CN-F(E) | Human Anti-HTRA1 Recombinant Antibody; Fab Fragment (HPAB-0530-CN-F(E)) | WB, ELISA, FC, IHC | Humanized Fab |
| HPAB-0400-YC-F(E) | Human Anti-HTRA1 Recombinant Antibody; Fab Fragment (HPAB-0400-YC-F(E)) | ELISA, FC, Block | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-3081LY-S(P) | Mouse Anti-HTRA1 Recombinant Antibody; scFv Fragment (HPAB-3081LY-S(P)) | ELISA, Block | Mouse scfv |
| HPAB-3082LY-S(P) | Mouse Anti-HTRA1 Recombinant Antibody; scFv Fragment (HPAB-3082LY-S(P)) | ELISA, Block | Mouse scfv |
| HPAB-3083LY-S(P) | Mouse Anti-HTRA1 Recombinant Antibody; scFv Fragment (HPAB-3083LY-S(P)) | ELISA, Block | Mouse scfv |
| HPAB-3084LY-S(P) | Mouse Anti-HTRA1 Recombinant Antibody; scFv Fragment (HPAB-3084LY-S(P)) | ELISA, Block | Mouse scfv |
| HPAB-3085LY-S(P) | Mouse Anti-HTRA1 Recombinant Antibody; scFv Fragment (HPAB-3085LY-S(P)) | ELISA, Block | Mouse scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC147 | Recombinant Anti-HTRA1 Eliminating Antibody, pH-Sensitive (VS-0625-YC147) | Antigen-Sweeping In Vivo. | Human IgG1 kappa |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: ELISA, FC
Application: Neut, FC
Application: ELISA
Application: ELISA, FC, FuncS
Application: ELISA, FC, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
