Rabbit Anti-ICAM3 Recombinant Antibody (clone R08-0D3)
CAT#: VS3-FY2012
Clone R08-0D3 is a rabbit recombinant monoclonal antibody that detects human ICAM3. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-P, IP. This ICAM3 antibody was verified by western blot analysis of ICAM3 in Hela, A549, HL-60, U2OS, C6 lysates and immunohistochemistry analysis of paraffin-embedded human tonsil.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nuclear membrane & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49820/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human bone marrow
High expression. RNA expression: 206.5 nTPM.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2498/6124_B_5_4_val_selected.jpg
Normal Tissue
Figure 3 IHC staining of human cerebral cortex
Low expression. RNA expression: 6.4 nTPM.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2498/6124_B_7_5_val_selected.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2498/6124_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2498/6124_A_8_5.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2498/6124_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (Karpas-707, NB-4, RPMI-8226, U-266/84, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000076662-ICAM3
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WB
Figure 1 Anti-ICAM3 Antibody (VS3-FY2012) in WB.
The ICAM3 expression in Hela, A549, HL-60, U2OS, C6 lysates were detected using the anti-ICAM3 by western blot assay.
IHC
Figure 2 Anti-ICAM3 Antibody (VS3-FY2012) in IHC.
Immunohistochemistry staining of paraffin-embedded human tonsil using the anti-ICAM3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide of human ICAM3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat ICAM3
- Species Reactivity
- Human, Rat
- Clone
- R08-0D3
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 60 kDa, while the observed molecular weight stands at 110-160 kDa.
- Related Disease
- Colon Carcinoma In Situ and Intestine Carcinoma In Situ
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Intercellular Adhesion Molecule 3
- Gene ID
- 3385
- UniProt ID
- P32942
- Sequence Similarities
- Belongs to the immunoglobulin superfamily. ICAM family.
- Cellular Localization
- Membrane
- Post Translation Modifications
- Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues. (P32942-ICAM3_HUMAN)
N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains. (P32942-ICAM3_HUMAN)
Glycosylation at Asn52, Asn84, Asn87, Asn101, Asn110, Asn134, Asn206, Asn264, Asn295, Asn308, Asn320, Asn363, Asn389, Asn453, and Asn457 (NX_P32942 [NX_P32942-1])
Modification sites at PhosphoSitePlus (P32942)
Glycosylation from GlyConnect ICAM3_HUMAN (286)
Glycosylation from GlyGen (P32942) 17 sites, 26 N-linked glycans (1 site), 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001307534.1; NP_001307535.1; NP_001307537.1; NP_001382303.1; NP_001382304.1
- Function
- ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2).
ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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