Rabbit Anti-ID1 Recombinant Antibody (clone 2C7)
CAT#: ZG-0093U
The purified Rabbit Anti-ID1 Recombinant Antibody (clone 2C7) displayed high specificity against the ID1 protein of human and mouse.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-ID1 Antibody (ZG-0093U) in WB
Positive WB detected in: 293T whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate 293 whole cell lysate, A549 whole cell lysate, U-251 whole cell lysate, Mouse Brain whole cell lysate. All lanes: Id1 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 17, 16 kDa
Observed band size: 26 kDa
IF
Figure 2 Rabbit Anti-ID1 Antibody (ZG-0093U) in IF
Immunofluorescence staining of Hela Cells with ZG-0093U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51456/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58518/if_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25915/64826_A_8_3.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25915/64826_A_4_6.jpg
Normal Tissue
Figure 5 Placenta
Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25915/64826_A_2_7.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (EFO-21, HaCaT, Hep G2, PC-3)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000125968-ID1
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Specifications
- Immunogen
- A synthesized peptide derived from human Id1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ID1
- Species Reactivity
- Human, Mouse
- Clone
- 2C7
- Applications
- ELISA, WB, IF
- Conjugate
- Unconjugated
- Related Disease
- Fibrodysplasia Ossificans Progressiva and Cataract 3, Multiple Types
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IF:1:20-1:200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ID1"
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|---|---|---|---|
| MOR-1729 | Rabbit Anti-Id1 Recombinant Antibody (clone DS1729AB) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0307-YC | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-2/#5-3) | ELISA, WB, IHC | Rabbit IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0307-YC-S(P) | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-2/#5-3); scFv Fragment | ELISA, WB, IHC | Rabbit scFv |
| HPAB-0309-YC-S(P) | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-1/#195-14); scFv Fragment | ELISA, WB, IHC | Rabbit scFv |
| HPAB-0418-CN-S(P) | Rabbit Anti-Id1 Recombinant Antibody (clone #BCH-1/#37-2); scFv Fragment | WB, ELISA, IHC | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0307-YC-F(E) | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-2/#5-3); Fab Fragment | ELISA, WB, IHC | Rabbit Fab |
| HPAB-0309-YC-F(E) | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-1/#195-14); Fab Fragment | ELISA, WB, IHC | Rabbit Fab |
| HPAB-0418-CN-F(E) | Rabbit Anti-Id1 Recombinant Antibody (clone #BCH-1/#37-2); Fab Fragment | WB, ELISA, IHC | Rabbit Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1086 | Anti-ID1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3378 | Anti-Mouse ID1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3377 | Anti-Human ID1 Immunohistochemistry Kit | IHC |
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