Rabbit Anti-ID1 Recombinant Antibody (clone R01-2A4) (CAT#: VS3-XY3390)
This product is a recombinant rabbit antibody binding to ID1 (Inhibitor of DNA binding 1), which regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer. It specifically recognizes the epitope of human ID1. The antibody also has cross-reactivity against mouse and rat ID1. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51456/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51456/if_selected.jpg
Figure 2 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58518/if_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58518/if_selected.jpg
Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25915/64826_A_8_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25915/64826_A_8_3.jpg
Figure 4 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25915/64826_A_4_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25915/64826_A_4_6.jpg
Figure 5 Placenta
(Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25915/64826_A_2_7.jpg
(Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25915/64826_A_2_7.jpg
Figure 6 RNA cell line category: Cell line enhanced (EFO-21, HaCaT, Hep G2, PC-3)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000125968-ID1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000125968-ID1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ID1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-2A4
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Mouth Disease; Colorectal Cancer
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- ID; bHLHb24
- Gene ID
- 3397
- UniProt ID
- P41134
- Long Name
- Inhibitor of DNA binding 1
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (P41134)
Modification sites at neXtProt (NX_P41134)
- Protein Refseq
- NP_002156.2
- Function
- Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity.
Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation.
Inhibits skeletal muscle and cardiac myocyte differentiation.
Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer (By similarity).
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "ID1"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3006z | Mouse Anti-ID1 Recombinant Antibody (clone 29D9) | WB, ELISA, IF, IHC, IP | Mouse IgG2a, κ |
| HPAB-0307-YC | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-2/#5-3) | ELISA, WB, IHC | Rabbit IgG |
| HPAB-0309-YC | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-1/#195-14) | ELISA, WB, IHC | Rabbit IgG |
| HPAB-0418-CN | Rabbit Anti-Id1 Recombinant Antibody (clone #BCH-1/#37-2) | WB, ELISA, IHC | Rabbit IgG |
| MRO-0808-CN | Rabbit Anti-ID1 Recombinant Antibody (clone JM92-13) | WB, IF, IHC, FC | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1729 | Rabbit Anti-Id1 Recombinant Antibody (clone DS1729AB) | WB, IHC | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0307-YC-S(P) | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-2/#5-3); scFv Fragment | ELISA, WB, IHC | Rabbit scFv |
| HPAB-0309-YC-S(P) | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-1/#195-14); scFv Fragment | ELISA, WB, IHC | Rabbit scFv |
| HPAB-0418-CN-S(P) | Rabbit Anti-Id1 Recombinant Antibody (clone #BCH-1/#37-2); scFv Fragment | WB, ELISA, IHC | Rabbit scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0307-YC-F(E) | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-2/#5-3); Fab Fragment | ELISA, WB, IHC | Rabbit Fab |
| HPAB-0309-YC-F(E) | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-1/#195-14); Fab Fragment | ELISA, WB, IHC | Rabbit Fab |
| HPAB-0418-CN-F(E) | Rabbit Anti-Id1 Recombinant Antibody (clone #BCH-1/#37-2); Fab Fragment | WB, ELISA, IHC | Rabbit Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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