Rabbit Anti-ID1 Recombinant Antibody (clone R01-2A4)
CAT#: VS3-XY3390
This product is a recombinant rabbit antibody binding to ID1 (Inhibitor of DNA binding 1), which regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer. It specifically recognizes the epitope of human ID1. The antibody also has cross-reactivity against mouse and rat ID1. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51456/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58518/if_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25915/64826_A_8_3.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25915/64826_A_4_6.jpg
Normal Tissue
Figure 5 Placenta
Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25915/64826_A_2_7.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (EFO-21, HaCaT, Hep G2, PC-3)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000125968-ID1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ID1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-2A4
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Mouth Disease; Colorectal Cancer
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- ID; bHLHb24
- Gene ID
- 3397
- UniProt ID
- P41134
- Long Name
- Inhibitor of DNA binding 1
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (P41134)
Modification sites at neXtProt (NX_P41134)
- Protein Refseq
- NP_002156.2
- Function
- Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity.
Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation.
Inhibits skeletal muscle and cardiac myocyte differentiation.
Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ID1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2314MZ | Recombinant Mouse Anti-Human ID1 Antibody (clone 3567D2b) | IHC-P, WB | Mouse antibody |
| HPAB-0307-YC | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-2/#5-3) | ELISA, WB, IHC | Rabbit IgG |
| HPAB-0418-CN | Rabbit Anti-Id1 Recombinant Antibody (clone #BCH-1/#37-2) | WB, ELISA, IHC | Rabbit IgG |
| MRO-0808-CN | Rabbit Anti-ID1 Recombinant Antibody (clone JM92-13) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0093U | Rabbit Anti-ID1 Recombinant Antibody (clone 2C7) | ELISA, WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1729 | Rabbit Anti-Id1 Recombinant Antibody (clone DS1729AB) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0307-YC-S(P) | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-2/#5-3); scFv Fragment | ELISA, WB, IHC | Rabbit scFv |
| HPAB-0309-YC-S(P) | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-1/#195-14); scFv Fragment | ELISA, WB, IHC | Rabbit scFv |
| HPAB-0418-CN-S(P) | Rabbit Anti-Id1 Recombinant Antibody (clone #BCH-1/#37-2); scFv Fragment | WB, ELISA, IHC | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0307-YC-F(E) | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-2/#5-3); Fab Fragment | ELISA, WB, IHC | Rabbit Fab |
| HPAB-0309-YC-F(E) | Rabbit Anti-ID1 Recombinant Antibody (clone #BCH-1/#195-14); Fab Fragment | ELISA, WB, IHC | Rabbit Fab |
| HPAB-0418-CN-F(E) | Rabbit Anti-Id1 Recombinant Antibody (clone #BCH-1/#37-2); Fab Fragment | WB, ELISA, IHC | Rabbit Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1086 | Anti-ID1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3378 | Anti-Mouse ID1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3377 | Anti-Human ID1 Immunohistochemistry Kit | IHC |
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