Rabbit Anti-ID3 Recombinant Antibody (clone BCH-4/#17-3); scFv Fragment (CAT#: HPAB-0280-YJ-S(P))
This product is a recombinant rabbit Antibody scFv Fragment that recognizes Human/Mouse ID3. It has no substantial cross-reactivity to other family Id proteins such as Id1, Id2, or Id4, or other endogenous proteins present in the cells that express Id3 protein. It is useful in immunochemical-based assays such as ELISA, western blot, and immunohistochemical staining.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21353/613_G9_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21353/613_G9_2_selected.jpg
Figure 2 IHC staining of human bone marrow
(Immunohistochemical staining of human bone marrow shows strong nuclear positivity in bone marrow poietic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24677/ihc_selected.jpg
(Immunohistochemical staining of human bone marrow shows strong nuclear positivity in bone marrow poietic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24677/ihc_selected.jpg
Figure 3 IHC staining of human gall bladder
(Immunohistochemical staining of human gall bladder shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21353/47677_A_6_4_selected.jpg
(Immunohistochemical staining of human gall bladder shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21353/47677_A_6_4_selected.jpg
Figure 4 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24677/53711_B_9_5.jpg
(Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24677/53711_B_9_5.jpg
Figure 5 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24677/53711_A_9_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24677/53711_A_9_3.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24677/53711_A_6_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24677/53711_A_6_6.jpg
Figure 7 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24677/53711_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/24677/53711_A_8_8.jpg
Figure 8 RNA cell line category: Cell line enhanced (U-698)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117318-ID3
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117318-ID3
Specifications
- Immunogen
- Recombinant hid3:GST
- Host Species
- Rabbit
- Type
- Rabbit scFv
- Specificity
- Human
- Species Reactivity
- Human, Mouse
- Clone
- BCH-4/#17-3
- Applications
- ELISA, WB, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- HEIR-1; bHLHb25; DNA-binding protein inhibitor ID-3; ID-like protein inhibitor HLH 1R21; class B basic helix-loop-helix protein 25; helix-loop-helix protein HEIR-1; inhibitor of DNA binding 3, dominant negative helix-loop-helix protein; inhibitor of differentiation 3
- Gene ID
- 3399
- UniProt ID
- Q02535
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
ScFv Antibody Products
Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2316MZ | Recombinant Mouse Anti-Human ID3 Antibody | ICC/IF, WB | Mouse antibody |
| HPAB-0278-YJ | Rabbit Anti-ID3 Recombinant Antibody (clone BCH-4/#3-3) | ELISA, WB, IHC | Rabbit IgG |
| HPAB-0279-YJ | Rabbit Anti-ID3 Recombinant Antibody (clone BCH-4/#16-1) | ELISA, WB, IHC | Rabbit IgG |
| HPAB-0280-YJ | Rabbit Anti-ID3 Recombinant Antibody (clone BCH-4/#17-3) | ELISA, WB, IHC | Rabbit IgG |
| VS3-FY720 | Recombinant Mouse Anti-ID3 Antibody (clone 4C8D8) | ELISA, IHC, FC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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