Mouse Anti-IGF1R Monoclonal Antibody (VS7-0425-WR118)

Name: Mouse Anti-IGF1R Monoclonal Antibody (VS7-0425-WR118)
SKU: VS7-0425-WR118
Rating: 5 (1 reviews)

CAT#: VS7-0425-WR118

This product is a mouse IgG antibody that binds to human EPAS1. The antibody 1H3E62 can be used for WB, IHC-P, IF, and ELISA assays.

Gene Expression

Normal Tissue Figure 1 Cerebral cortex

Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10268/32662_B_7_5.jpg

Normal Tissue Figure 2 Colon

Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10268/32662_A_7_3.jpg

Normal Tissue Figure 3 Liver

Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10268/32662_A_8_4.jpg

Normal Tissue Figure 4 Kidney

Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10268/32662_A_7_5.jpg

Normal Tissue Figure 5 Testis

Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10268/32662_A_6_6.jpg

Normal Tissue Figure 6 Heart muscle

Cardiomyocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10268/32662_B_5_6.jpg

Normal Tissue Figure 7 Lymph node

Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10268/32662_A_7_8.jpg

RNA Expression Figure 8 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000140443-IGF1R

❮

❯

Specifications

Immunogen
Recombinant human IGF1R fragment expressed in E. Coli.

Host Species
Mouse

Type
Mouse IgG

Specificity
Human IGF1R

Species Reactivity
Human

Molecule Class
Receptor Tyrosine Kinase

Applications
WB, IHC-P, IF, ELISA

Conjugate
Unconjugated

Related Disease
Insulin-like growth factor 1 resistance (IGF1RES)

Product Property

Clonality
Monoclonal

Purity
>95% as determined by SDS-PAGE and SEC-HPLC analysis

Format
Liquid

Concentration
1 mg/mL

Preservative
0.03% sodium azide

Storage
Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Immunohistochemistry: 1:200 - 1:1000

Target

Alternative Names
IGFR; CD221; IGFIR; JTK13

Gene ID
3480

UniProt ID
P08069

Long Name
Insulin Like Growth Factor 1 Receptor

Cellular Localization
Cell membrane; Membrane

Post Translation Modifications
Cleavage on pair of basic residues; Disulfide bond; Glycoprotein; Isopeptide bond; Phosphoprotein; Ubl conjugation

Protein Refseq
NP_000866.1

Function
Kinase; Receptor; Transferase; Tyrosine-protein kinase

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Related Signaling Pathways

Insulin Signaling Pathway

Related Diseases

Endocrine Resistance

Type I Diabetes Mellitus

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS7-0425-WR118.pdf

MSDS

MSDS - VS7-0425-WR118.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

CAT
Product Name

Secondary Antibody

CAT
Product Name

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HPAB-0211CQ-F(E)	Human Anti-IGF1R Recombinant Antibody (clone 1H3); Fab Fragment	ELISA, FC, Neut	Human Fab
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