Mouse Anti-IGFBP2 Monoclonal Antibody (VS7-0425-WR120)
CAT#: VS7-0425-WR120
This product is a mouse IgG antibody that binds to human EPAS1. The antibody 1H3E62 recognizes a specific amino acid domains of IGFBP2 (aa180-328). It can be used for WB, IHC-P, IF, and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/77723/1615_H9_6_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (ASC diff, HMC-1, NB-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115457-IGFBP2
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Specifications
- Immunogen
- Recombinant IGFBP2 (aa180-328) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human IGFBP2
- Species Reactivity
- Human
- Molecule Class
- Binding Protein
- Applications
- WB, IHC-P, IF, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Glioblastoma; Ovarian cancer; Prostate cancer; Type 2 diabetes
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/mL
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Immunohistochemistry: 1:200 - 1:1000
Target
- Alternative Names
- IBP2; IGF-BP53
- Gene ID
- 3485
- UniProt ID
- P18065
- Long Name
- Insulin Like Growth Factor Binding Protein 2
- Cellular Localization
- Secreted
- Post Translation Modifications
- Disulfide bond; Glycoprotein
- Protein Refseq
- NP_000588.3
- Function
- Growth factor binding
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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