A antibody, 12.5H, was selected because of its ability to recoginize, in ELISA, IgG mAb with DNA-binding activity. mAb 12.5H binds perferentlly to IgG2a mAb and does not react with IgG2a mAb with no DNA-binding activity.
Figure 1 Interactions of 12.5H with IgG antibodies purified from B/W or BALB/C mice.
ELISA of mAb 12.5H.
Figure 2 Western blot analysis of 12.5 H using mAb 4B1.
Mab 12.5 H and IgG anti-DNA antibodies interact through their variable regions.
Figure 3 Indirect immunofluorescence study of 12.5 H.
Ethanol-fixed hep-2 cells were incubated with Mab 12.5 H, washed and incubated with fluorescein -conjugated goat anti-mouse Ig.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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FAMAB-0177-YC-S(P) | Mouse Anti-IgG Recombinant Antibody (clone 17F12); scFv Fragment | ELISA | Mouse scFv |
FAMAB-0178-YC-S(P) | Mouse Anti-IgG Recombinant Antibody (clone 12.5H); scFv Fragment | ELISA, IF, WB | Mouse scFv |
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NABG-092 | Recombinant Anti-Pig IGG VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
NABC-001 | Recombinant Anti-Rabbit IgG VHH Single Domain Antibody [Magnetic Beads] | IP | Llama VHH |
NABC-007 | Recombinant Anti-Rabbit IgG VHH Single Domain Antibody [Sepharose] | IP | Llama VHH |
NABC-008 | Recombinant Anti-Rabbit IgG VHH Single Domain Antibody [HRP] | WB, IHC-P, ELISA | Llama VHH |
HPAB-0727-YJ-VHH | Camelid Anti-Pig IgG Recombinant Single Domain Antibody (VI-16) | ELISA, WB, In vivo stable auxiliary | Camelid VHH |
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MOB-0011MZ | Recombinant Mouse Anti-Armenian Hamster IgG1 Antibody (clone TB140a) | ELISA | Mouse antibody |
MOB-0012MZ | Recombinant Mouse Anti-Armenian Hamster IgG2/IgG3 Antibody (clone TB140e) | ELISA | Mouse antibody |
MOB-0014MZ | Recombinant Mouse Anti-Armenian Hamster IgG3 Antibody (clone TB140b) | ELISA | Mouse antibody |
MOB-0015MZ | Recombinant Mouse Anti-Monkey IgG Antibody (clone TB109a) | ELISA | Mouse antibody |
CBPAB-063LC | Goat Anti-Mouse IgG Polyclonal Antibody, Alexa 488 | ELISA, IHC, ICC, FC | Goat IgG |
CAT | Product Name | Application | Type |
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BRD-0485MZ | Chicken Anti-Rabbit IgG Polyclonal IgY | WB | Chicken antibody |
CAT | Product Name | Application | Type |
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MOR-1764 | Rabbit Anti-IgG Recombinant Antibody (clone DS1764AB) | IHC-P | Rabbit IgG |
CAT | Product Name | Application | Type |
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FAMAB-0177-YC-F(E) | Mouse Anti-IgG Recombinant Antibody (clone 17F12); Fab Fragment | ELISA | Mouse Fab |
FAMAB-0178-YC-F(E) | Mouse Anti-IgG Recombinant Antibody (clone 12.5H); Fab Fragment | ELISA, IF, WB | Mouse Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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