Mouse Anti-IgG Recombinant Antibody (clone 12.5H) (CAT#: FAMAB-0178YC)
A antibody, 12.5H, was selected because of its ability to recoginize, in ELISA, IgG mAb with DNA-binding activity. mAb 12.5H binds perferentlly to IgG2a mAb and does not react with IgG2a mAb with no DNA-binding activity.
Specific Inquiry
ELISA
Figure 1 Interactions of 12.5H with IgG antibodies purified from B/W or BALB/C mice.
ELISA of mAb 12.5H.
WB
Figure 2 Western blot analysis of 12.5 H using mAb 4B1.
Mab 12.5 H and IgG anti-DNA antibodies interact through their variable regions.
IF
Figure 3 Indirect immunofluorescence study of 12.5 H.
Ethanol-fixed hep-2 cells were incubated with Mab 12.5 H, washed and incubated with fluorescein -conjugated goat anti-mouse Ig.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgM
- Specificity
- Mouse IgG
- Species Reactivity
- Mouse
- Clone
- 12.5H
- Applications
- ELISA, IF, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- IgG; Immunoglobulin G
Related Resources
Rheumatoid Arthritis
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-950 | Human Anti-IgG Recombinant Antibody (clone YC176); scFv Fragment | ELISA | Human scFv |
| FAMAB-0177-YC-S(P) | Mouse Anti-IgG Recombinant Antibody (clone 17F12); scFv Fragment | ELISA | Mouse scFv |
| FAMAB-0178-YC-S(P) | Mouse Anti-IgG Recombinant Antibody (clone 12.5H); scFv Fragment | ELISA, IF, WB | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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