Mouse Anti-IgG Recombinant Antibody (clone 12.5H) (CAT#: FAMAB-0178YC)

A antibody, 12.5H, was selected because of its ability to recoginize, in ELISA, IgG mAb with DNA-binding activity. mAb 12.5H binds perferentlly to IgG2a mAb and does not react with IgG2a mAb with no DNA-binding activity.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Datasheet
  • MSDS
  • COA
ELISA

Figure 1 Interactions of 12.5H with IgG antibodies purified from B/W or BALB/C mice.

Figure 1 Interactions of 12.5H with IgG antibodies purified from B/W or BALB/C mice.

ELISA of mAb 12.5H.

WB

Figure 2 Western blot analysis of 12.5 H using mAb 4B1.

Figure 2 Western blot analysis of 12.5 H using mAb 4B1.

Mab 12.5 H and IgG anti-DNA antibodies interact through their variable regions.

IF

Figure 3 Indirect immunofluorescence study of 12.5 H.

Figure 3 Indirect immunofluorescence study of 12.5 H.

Ethanol-fixed hep-2 cells were incubated with Mab 12.5 H, washed and incubated with fluorescein -conjugated goat anti-mouse Ig.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgM
  • Specificity
  • Mouse IgG
  • Species Reactivity
  • Mouse
  • Clone
  • 12.5H
  • Applications
  • ELISA, IF, WB

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • IgG; Immunoglobulin G

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone 12.5H"

See other products for "IgG"

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0485MZ Chicken Anti-Rabbit IgG Polyclonal IgY WB Chicken antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1764 Rabbit Anti-IgG Recombinant Antibody (clone DS1764AB) IHC-P Rabbit IgG

Customer Reviews and Q&As

Submit a review or a question
There are currently no Customer reviews or questions for FAMAB-0178YC. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart

    Go to compare