Mouse Anti-IGHA1 Recombinant Antibody (clone 7D5F12)

CAT#: VS7-0425-WR122

This product is a mouse IgG1 recombinant antibody that binds to human IGHA1. The antibody 7D5F12 recognizes a specific amino acid domain of human IghA1 (AA: 207-353). It can be used for WB, IHC, ICC, and FC assays. It cross reatcs with mouse and monkey.

Gene Expression
Figure 1 RNA cell line category: Cell line enhanced (HDLM-2, RPMI-8226, SH-SY5Y, U-266/70, U-266/84)

Specifications

  • Immunogen
  • Recombinant human IghA1 (AA: 207-353) fragment expressed in E. Coli.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Human IGHA1
  • Species Reactivity
  • Human, Mouse, Monkey
  • Clone
  • 7D5F12
  • Molecule Class
  • Immunoglobulin
  • Applications
  • WB, IHC, ICC, FC
  • Conjugate
  • Unconjugated
  • Related Disease
  • IgA nephropathy; Various mucosal infections; Henoch-Schönlein purpura

Product Property

  • Clonality
  • Monoclonal
  • Purity
  • >95% as determined by SDS-PAGE and SEC-HPLC analysis
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • 0.05% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • Western Blot: 1:500 - 1:2000
    Immunohistochemistry-Paraffin: 1:200 - 1:1000
    Immunocytochemistry: 1:50 - 1:250
    Flow Cytometry: 1:200 - 1:400
    Enzyme-linked Immunosorbent Assay: 1:10000

Target

  • Alternative Names
  • IgA1
  • Long Name
  • Immunoglobulin Heavy Constant Alpha 1
  • Cellular Localization
  • Cell membrane; Immunoglobulin; Membrane; Secreted
  • Post Translation Modifications
  • Disulfide bond; Glycoprotein
  • Function
  • Immunity
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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

Certificate of Analysis Lookup

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

Secondary Antibody

Reconstitution Buffer

Recommended Dilution Buffer

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