Mouse Anti-IKBKB Monoclonal Antibody (VS7-0425-WR127)
CAT#: VS7-0425-WR127
This product is a mouse IgG antibody that binds to human IKBKB. The antibody can be used for WB, IHC-P, IF, and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1249/if_selected.jpg
Normal Tissue
Figure 2 Cerebellum.
High expression in cerebellum
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4447/12997_B_8_8_val_selected.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4447/12997_A_6_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4447/12997_A_7_8.jpg
Normal Tissue
Figure 5 Parathyroid gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4447/12997_A_3_8.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4447/12997_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4447/12997_A_8_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104365-IKBKB
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Specifications
- Immunogen
- Recombinant human IKBKB fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human IKBKB
- Species Reactivity
- Human
- Molecule Class
- Kinase
- Applications
- WB, IHC-P, IF, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Certain immune deficiencies; Increased susceptibility to inflammatory diseases.
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/mL
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- IKK2; IKKB; IKK-2; IMD15; IMD15A; IMD15B; NFKBIKB; IKK-beta
- Gene ID
- 3551
- UniProt ID
- O14920
- Long Name
- Inhibitor Of Nuclear Factor Kappa B Kinase Subunit Beta
- Cellular Localization
- Cytoplasm; Membrane; Nucleus
- Post Translation Modifications
- Acetylation; Hydroxylation; Isopeptide bond; Phosphoprotein; S-nitrosylation; Ubl conjugation
- Protein Refseq
- NP_001177649.1
- Function
- ATP binding; IkappaB kinase activity; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity;
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "IKBKB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0274MZ | Chicken Anti-IKBKB Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1781 | Rabbit Anti-IKBKB Recombinant Antibody (clone DS1781AB) | WB, IHC, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1960-CN | Rabbit Anti-IKBKB Polyclonal Antibody (MRO-1960-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0763J | Rabbit Anti-IKBKB Recombinant Antibody (clone 5D12) | ELISA, WB | Rabbit IgG |
| ZG-0764J | Rabbit Anti-IKBKB Recombinant Antibody (clone 3E1) | ELISA, WB, IHC, IF | Rabbit IgG |
| VS3-XY851 | Mouse Anti-IKBKB Recombinant Antibody (clone 10A2) | ELISA, WB | Mouse IgG1 |
| VS3-WK1362 | Mouse Anti-IKBKB Recombinant Antibody (VS3-WK1362) | IHC-p | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY146 | AbPlus™ Anti-IKBKB Magnetic Beads (VS-0424-XY146) | IP, Protein Purification | |
| VS-0724-YC981 | AbPlus™ Anti-IKBKB Magnetic Beads (VS-0724-YC981) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY275 | Rabbit Anti-NHP IKBKB Recombinant Antibody (VS-1024-XY275) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1113 | Anti-IKBKB Immunohistochemistry Kit | IHC | |
| VS-0525-XY3470 | Anti-Mouse IKBKB Immunohistochemistry Kit | IHC | |
| VS-0525-XY3469 | Anti-Human IKBKB Immunohistochemistry Kit | IHC |
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