Mouse Anti-IL16 Monoclonal Antibody (clone 5D8) (CAT#: VS7-0425-WR133)
This product is a mouse IgG1 antibody that binds to human IL16. The antibody 5D8 can be used for WB and ELISA assays.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEL
(Immunofluorescent staining of human cell line HEL shows localization to nuclear speckles & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18467/1961_C5_1_selected.jpg
(Immunofluorescent staining of human cell line HEL shows localization to nuclear speckles & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18467/1961_C5_1_selected.jpg
Figure 2 IF staining of human cell line HEL
(Immunofluorescent staining of human cell line HEL shows localization to nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48861/1770_D10_3_selected.jpg
(Immunofluorescent staining of human cell line HEL shows localization to nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48861/1770_D10_3_selected.jpg
Figure 3 Tonsil
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18467/50106_A_4_8.jpg
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18467/50106_A_4_8.jpg
Figure 4 Lymph node
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18467/50106_A_7_8.jpg
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18467/50106_A_7_8.jpg
Figure 5 RNA cell line category: Cell line enhanced (Daudi, HEL, HMC-1, RPMI-8226, U-266/70, U-698, U-937)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172349-IL16
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172349-IL16
Specifications
- Immunogen
- Recombinant human IL-16 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human IL-16
- Species Reactivity
- Human
- Clone
- 5D8
- Molecule Class
- Cytokines
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Asthma; HIV/AIDS; Various inflammatory conditions.
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- LCF; NIL16; PRIL16; prIL-16
- Gene ID
- 3603
- UniProt ID
- Q14005
- Long Name
- Interleukin 16
- Cellular Localization
- Cytoplasm, Nucleus, Secreted
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_001165599.1
- Function
- Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2138z | Mouse Anti-IL16 Recombinant Antibody (clone 27G12) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| MOB-1195MZ | Recombinant Mouse Anti-Human IL16 Antibody (clone 60) | ELISA, WB | Mouse antibody |
| ZG-0495J | Mouse Anti-IL16 Recombinant Antibody (ZG-0495J) | WB, ELISA | Mouse IgG |
| VS3-XY855 | Mouse Anti-IL16 Recombinant Antibody (clone 5D8) | ELISA, WB | Mouse IgG1 |
| VS3-WK1718 | Recombinant Rabbit Anti-IL16 Capture Antibody (clone R04-6D1) | ELISA | Rabbit IgG |
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