Mouse Anti-IL17RB Recombinant Antibody; Fab Fragment (HPAB-AP684-YC-F(E)) (CAT#: HPAB-AP684-YC-F(E))
This product is a recombinant mouse antibody Fab fragment that recognizes IL17RB. The antibody was purified by affinity chromatography.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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DB
-1.jpg)
Figure 1 Mouse Anti-IL17RB Recombinant Antibody (HPAB-AP684-YC-F(E)) in DB
Dot Blot analysis of HPAB-AP684-YC-F(E) was performed by coating with human IL17RB protein (His tag).
HPAB-AP684-YC-F(E) incubation concentration: 2 μg/mL.
HRP conjugated Anti-His tag as a secondary antibody (1: 2000)
WB
-2.jpg)
Figure 2 Mouse Anti-IL17RB Recombinant Antibody (HPAB-AP684-YC-F(E)) in WB
Western blot analysis of HPAB-AP684-YC-F(E) was performed by loading human IL17RB protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-AP684-YC-F(E) and HRP conjugated Anti-His tag as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
ELISA
-3.jpg)
Figure 3 Mouse Anti-IL17RB Recombinant Antibody (HPAB-AP684-YC-F(E)) in ELISA
ELISA analysis of HPAB-AP684-YC-F(E) was performed by coating with human IL17RB protein (His tag). Then blocked with BSA and incubated with HPAB-AP684-YC-F(E). The HRP conjugated Anti-His tag as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Mouse
- Type
- Mouse Fab
- Specificity
- Human IL17RB
- Species Reactivity
- Human, Mouse
- Applications
- ELISA, FC (1 μg/ml), Inhib, FuncS
- Related Disease
- Asthma, Ulcerative colitis and Crohn's disease
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- See the COA
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Single Domain Antibody Products
IgG Antibody Products
Neutralizing Antibody Products
ScFv Antibody Products
Fab Antibody Products
Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1426-VHH | Recombinant Anti-Human IL17RB VHH Single Domain Antibody | WB, IP, ICC, FC, FA | Llama VHH |
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