Human Anti-IL2 Recombinant Antibody (PABC-506) (CAT#: PABC-506)
This product is a recombinant Human antibody that can recognize IL2.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
ELISA
Figure 1 Anti-IL2 Recombinant Antibody (PABC-506) in ELISA
ELISA analysis of PABC-506 was performed by coating with human IL2 protein (His tag). Then blocked with BSA and incubated with PABC-506. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Anti-IL2 Recombinant Antibody (PABC-506) in WB
Western blot analysis of PABC-506 was performed by loading human IL2 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PABC-506 and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.
DB
Figure 3 Anti-IL2 Recombinant Antibody (PABC-506) in DB
Dot Blot analysis of PABC-506 was performed by coating with human IL2 protein (His tag).
PABC-506 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control
Specifications
- Host Species
- Human
- Type
- Human IgG1, λ
- Specificity
- Human IL2
- Species Reactivity
- Human
- Applications
- Inhib, ELISA, FC, FuncS
- Related Disease
- Autoimmune diseases and graft-versus-host disease
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Inhibition, Flow Cytometry and Functional Assay. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
Related Resources
Autoimmune Thyroid Disease
Maturity Onset Diabetes of the Young
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-093 | Recombinant Anti-Mouse Il2 VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABC-506, RRID: AB_3111595)
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For Research Use Only. Not For Clinical Use.
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