Human Anti-IL20 Recombinant Antibody (clone 15D2) (CAT#: HPAB-0723-CN)

This is an anti-human IL20 monoclonal antibody that can reduce IL20 mediated activation of both IL20R1/IL20R2 and IL22R1/IL20R2 receptor complexes. 15D2 inhibited cynomolgus IL20-induced as well as mouse IL20-induced proliferation of the BaF-3(hIL20R) cells, while hIL19 or hIL24-induced proliferation of BaF-3(hIL20R) was not inhibited by 15D2.


Specific Inquiry
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  • Purity:
  • Fc Engineering:
  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
WB

Figure 1 IL-20 induction of tumor necrosis factor α (TNFα) in SFs from rats with CIA was determined at the indicated time points.

Figure 1 IL-20 induction of tumor necrosis factor α (TNFα) in SFs from rats with CIA was determined at the indicated time points.

Functions of interleukin-20 (IL-20) in synovial fibroblasts (SFs).

Hsu, Y. H., & Chang, M. S. (2010). Interleukin‐20 antibody is a potential therapeutic agent for experimental arthritis. Arthritis & Rheumatism, 62(11), 3311-3321.

FC

Figure 2 Induction of RANKL was measured in untreated MC3T3-E1 cells and in MC3T3-E1 cells treated for 16 hours with IL-20 (200 ng/ml), IL-17 (50 ng/ml), or both IL-20 and IL-27.

Figure 2 Induction of RANKL was measured in untreated MC3T3-E1 cells and in MC3T3-E1 cells treated for 16 hours with IL-20 (200 ng/ml), IL-17 (50 ng/ml), or both IL-20 and IL-27.

Cells were stained with phycoerythrin-conjugated anti-RANKL antibody and analyzed by flow cytometry. Values are the mean and SD.

Hsu, Y. H., & Chang, M. S. (2010). Interleukin‐20 antibody is a potential therapeutic agent for experimental arthritis. Arthritis & Rheumatism, 62(11), 3311-3321.

IHC

Figure 3 Immunohistochemical stain of skin section with monoclonal antibodies against IL-20.

Figure 3 Immunohistochemical stain of skin section with monoclonal antibodies against IL-20.

Strong expression of IL-20 in some perivascular mononuclear cells (could be dendritic cells).

Wei, C. C., Chen, W. Y., Wang, Y. C., Chen, P. J., Lee, J. Y. Y., Wong, T. W., ... & Lin, Y. C. (2005). Detection of IL-20 and its receptors on psoriatic skin. Clinical Immunology, 117(1), 65-72.

ELISA

Figure 4 IL-20 level in the blood of psoriasis patients and healthy control.

Figure 4 IL-20 level in the blood of psoriasis patients and healthy control.

Horizontal bars show mean values of IL-20 concentration in serum of healthy controls (215 ng/ml) and psoriasis patients (67.2 ng/ml).

Wei, C. C., Chen, W. Y., Wang, Y. C., Chen, P. J., Lee, J. Y. Y., Wong, T. W., ... & Lin, Y. C. (2005). Detection of IL-20 and its receptors on psoriatic skin. Clinical Immunology, 117(1), 65-72.


Specifications

  • Immunogen
  • Human IL-20
  • Host Species
  • Human
  • Type
  • Human IgG4, κ
  • Specificity
  • Human IL20
  • Species Reactivity
  • Human, Cynomolgus, Mouse
  • Clone
  • 15D2
  • Applications
  • WB, ELISA, FC, IHC
  • Related Disease
  • Autoimmune, Inflammatory diseases

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The IL20 antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Flow Cytometry.

Target

  • Alternative Names
  • Interleukin 20

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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Humanized Antibody

Human Antibody

Neutralizing Antibody

CAT Product Name Application Type
NEUT-1324CQ Rat Anti-Il20 Recombinant Antibody (clone CBL626) Neut Rat IgG2b

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-4256 Rabbit Anti-IL20 Recombinant Antibody (clone SI336DS) ELISA Rabbit IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-H34 Afuco™ Anti-IL20 ADCC Recombinant Antibody (Fletikumab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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