Rabbit Anti-Il33 Recombinant Antibody (clone R75-9F-3)
CAT#: VS3-FY2182
This R75-9F-3 recombinant monoclonal antibody specifically attaches to mouse Il33 protein, a calculated weight of 30 kDa. It cross-reacts with rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R75-9F-3 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200), immunofluorescence (dilution range 1:50-1:200).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line EFO-21
Immunofluorescent staining of human cell line EFO-21 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22899/1907_B1_4_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows nuclear positivity in endothelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/24426/53688_A_7_3_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22899/1936_B1_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22899/1953_C1_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7057/18488_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7057/18488_A_7_3.jpg
Normal Tissue
Figure 7 Endometrium
Cells in endometrial stroma
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7057/18488_B_4_3.jpg
Normal Tissue
Figure 8 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7057/18488_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Group enriched (hTERT-RPE1, U-2197)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000137033-IL33
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Mouse and Rat Il33
- Species Reactivity
- Mouse, Rat
- Clone
- R75-9F-3
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 30kDa, while the observed molecular weight stands at 33kDa.
- Related Disease
- Allergy-related diseases
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Interleukin 33
- Gene ID
- 77125
- UniProt ID
- O95760
- Sequence Similarities
- Belongs to the IL-1 family. Highly divergent.
- Cellular Localization
- Chromosome, Cytoplasm, Cytoplasmic vesicle, Nucleus, Secreted
- Post Translation Modifications
- The full-length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CELA1, CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein.
- Protein Refseq
- NP_001186569.1
- Function
- Acts as an enhancer of polarization of alternatively activated macrophages.
Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Il33"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-769-sdAb | Recombinant Anti-Mouse Il33 VHH Single Domain Antibody | ICC, ChiP, FC, FA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0454CL-S(P) | Human Anti-IL33 Recombinant Antibody; scFv Fragment (TAB-0454CL-S(P)) | Neut, FuncS | Human scFv |
| TAB-0455CL-S(P) | Human Anti-IL33 Recombinant Antibody; scFv Fragment (TAB-0455CL-S(P)) | Neut, FuncS | Human scFv |
| TAB-0456CL-S(P) | Human Anti-IL33 Recombinant Antibody; scFv Fragment (TAB-0456CL-S(P)) | Neut, FuncS | Human scFv |
| TAB-0457CL-S(P) | Human Anti-IL33 Recombinant Antibody; scFv Fragment (TAB-0457CL-S(P)) | Neut, FuncS | Human scFv |
| TAB-0458CL-S(P) | Human Anti-IL33 Recombinant Antibody; scFv Fragment (TAB-0458CL-S(P)) | Neut, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-657LC | Mouse Anti-IL33 Recombinant Antibody (TAB-657LC) | ELISA, FC | Mouse IgG1 |
| TAB-658LC | Mouse Anti-IL33 Recombinant Antibody (TAB-658LC) | ELISA, FC | Mouse IgG1 |
| TAB-657LC-S(P) | Mouse Anti-IL33 Recombinant Antibody; scFv Fragment (TAB-657LC-S(P)) | ELISA, FC | Mouse scFv |
| TAB-658LC-S(P) | Mouse Anti-IL33 Recombinant Antibody; scFv Fragment (TAB-658LC-S(P)) | ELISA, FC | Mouse scFv |
| TAB-657LC-F(E) | Mouse Anti-IL33 Recombinant Antibody; Fab Fragment (TAB-657LC-F(E)) | ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1386CQ | Mouse Anti-IL33 Recombinant Antibody (NEUT-1386CQ) | Neut | Mouse IgG1 |
| NEUT-1387CQ | Goat Anti-IL33 Recombinant Antibody (clone CBL774) | WB, IHC, Neut | Goat IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1811 | Rabbit Anti-IL33 Recombinant Antibody (clone DS1811AB) | FC | Rabbit IgG |
| MOR-4268 | Rabbit Anti-IL33 Recombinant Antibody (clone SI350DS) | FC | Rabbit IgG |
| MOR-4618 | Rabbit Anti-Il33 Recombinant Antibody (clone TH131DS) | WB | Rabbit IgG |
| MOR-4619 | Rabbit Anti-IL33 Recombinant Antibody (clone TH132DS) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0320-CN | Mouse Anti-IL33 Recombinant Antibody (HPAB-0320-CN) | IHC | Mouse IgG2a, κ |
| HPAB-0321-CN | Mouse Anti-IL33 Recombinant Antibody (HPAB-0321-CN) | ELISA, FC, IHC | Mouse IgG2a, κ |
| HPAB-0322-CN | Human Anti-IL33 Recombinant Antibody (HPAB-0322-CN) | WB, ELISA, IHC, FC, Block | Humanized IgG4 |
| HPAB-1206LY | Human Anti-IL33 Recombinant Antibody (HPAB-1206LY) | Neut, ELISA | Human IgG |
| HPAB-1207LY | Human Anti-IL33 Recombinant Antibody (HPAB-1207LY) | Neut, ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1206LY-S(P) | Human Anti-IL33 Recombinant Antibody; scFv Fragment (HPAB-1206LY-S(P)) | Neut, ELISA | Human scfv |
| HPAB-1207LY-S(P) | Human Anti-IL33 Recombinant Antibody; scFv Fragment (HPAB-1207LY-S(P)) | Neut, ELISA | Human scfv |
| HPAB-1208LY-S(P) | Human Anti-IL33 Recombinant Antibody; scFv Fragment (HPAB-1208LY-S(P)) | Neut, ELISA | Human scfv |
| HPAB-1209LY-S(P) | Human Anti-IL33 Recombinant Antibody; scFv Fragment (HPAB-1209LY-S(P)) | Neut, ELISA | Human scfv |
| HPAB-1210LY-S(P) | Human Anti-IL33 Recombinant Antibody; scFv Fragment (HPAB-1210LY-S(P)) | Neut, ELISA | Human scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1221LY-F(E) | Human Anti-IL33 Recombinant Antibody; Fab Fragment (HPAB-1221LY-F(E)) | Neut, ELISA | Human Fab |
| HPAB-1222LY-F(E) | Human Anti-IL33 Recombinant Antibody; Fab Fragment (HPAB-1222LY-F(E)) | Neut, ELISA | Human Fab |
| HPAB-1223LY-F(E) | Human Anti-IL33 Recombinant Antibody; Fab Fragment (HPAB-1223LY-F(E)) | Neut, ELISA | Human Fab |
| HPAB-1224LY-F(E) | Human Anti-IL33 Recombinant Antibody; Fab Fragment (HPAB-1224LY-F(E)) | Neut, ELISA | Human Fab |
| HPAB-1225LY-F(E) | Human Anti-IL33 Recombinant Antibody; Fab Fragment (HPAB-1225LY-F(E)) | Neut, ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY37 | Human Anti-IL33 (clone W25scFv88) scFv-Fc Chimera | IA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3528 | Anti-IL33 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC187 | Recombinant Anti-IL33 Eliminating Antibody, pH-Sensitive (VS-0625-YC187) | Antigen-Sweeping In Vivo. | Human IgG4 |
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