Mouse Anti-IL3RA Monoclonal Antibody (clone 10B8E7)
CAT#: VS7-0425-WR140
This product is a mouse IgG1 antibody that binds to human IL3RA. The antibody 10B8E7 recognizes a specific amino acid domain of human IL3RA (AA: 200-305). It can be used for ICC and FC assays.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_A_5_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HDLM-2, NB-4, RPMI-8226)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185291-IL3RA
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Specifications
- Immunogen
- Recombinant human IL3RA (AA: 200-305) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human IL3RA
- Species Reactivity
- Human
- Clone
- 10B8E7
- Molecule Class
- Interleukin-3 Receptor
- Applications
- ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Leukemia
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra; IL-3R-alpha
- Gene ID
- 3563
- UniProt ID
- P26951
- Long Name
- Interleukin 3 Receptor Subunit Alpha
- Cellular Localization
- Membrane
- Post Translation Modifications
- Disulfide bond; Glycoprotein; Ubl conjugation
- Protein Refseq
- NP_001254642
- Function
- This is a receptor for interleukin-3.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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