CytoStream™ Mouse Anti-IL3RA Recombinant Antibody (VS-0225-XY139)

Name: CytoStream™ Mouse Anti-IL3RA Recombinant Antibody (VS-0225-XY139)
SKU: VS-0225-XY139
Rating: 5 (1 reviews)

CAT#: VS-0225-XY139

This mouse-derived antibody targets the human IL3RA (Interleukin-3 Receptor Alpha) and is an essential tool for investigating immune signaling. It exhibits excellent reactivity with human samples, ensuring accurate results in various assays. Suitable for Flow Cytometry (FC), Immunofluorescence (IF), and Western Blot (WB), this antibody provides researchers with a robust solution for studying IL3RA's role in immune regulation and its potential in therapeutic applications.

Gene Expression

Normal Tissue Figure 1 IHC staining of human small intestine

Immunohistochemical staining of human small intestine shows cytoplasmic and membranous positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/ihc_selected.jpg

Normal Tissue Figure 2 Cerebral cortex

Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_B_8_5.jpg

Normal Tissue Figure 3 Colon

Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_A_9_3.jpg

Normal Tissue Figure 4 Kidney

Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_A_8_5.jpg

Normal Tissue Figure 5 Testis

Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_A_5_6.jpg

Normal Tissue Figure 6 Lymph node

Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_A_9_8.jpg

RNA Expression Figure 7 RNA cell line category: Cell line enhanced (HDLM-2, NB-4, RPMI-8226)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185291-IL3RA

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Specifications

Immunogen
Human IL3RA Transfected Cell Line

Host Species
Mouse

Type
Mouse IgG1, kappa

Specificity
IL3RA

Species Reactivity
Human

Applications
FC, IF, WB

Product Property

Purification
Protein A purified

Purity
>95%, determined by SDS-PAGE

Format
Liquid

Buffer
PBS

Preservative
Sodium azide

Storage
Store at 4°C for short-term storage and at -20°C for long-term storage. Avoid repeated freeze-thaw cycles.

Target

Alternative Names
IL3RA; IL-3RA; IL-3Rα; IL-3R-alpha; Interleukin-3 receptor subunit alpha

Gene ID
3563

UniProt ID
P26951

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-0225-XY139.pdf

MSDS

MSDS - VS-0225-XY139.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Recommended Dilution Buffer

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Product Name

Secondary Antibody

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