Rabbit Anti-ILK Recombinant Antibody (clone 6A5) (CAT#: ZG-0767J)
This product is a rabbit antibody that recognizes ILK.
Specific Inquiry
WB
Figure 1 Rabbit Anti-ILK Antibody (ZG-0767J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, HL60 whole cell lysate, A549 whole cell lysate, Mouse kidney tissue
All lanes: ILK antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52, 45, 37 kDa
Observed band size: 51 kDa
IF
Figure 2 Rabbit Anti-ILK Antibody (ZG-0767J) in IF
Immunofluorescence staining of MCF7 Cells with this product at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
IP
Figure 3 Rabbit Anti-ILK Antibody (ZG-0767J) in IP
Immunoprecipitating ILK in Hela whole cell lysate
Lane 1: Rabbit control IgG in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0767J (2µg)+ Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)
Specifications
- Immunogen
- A synthesized peptide derived from human ILK
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ILK
- Species Reactivity
- Human, Mouse
- Clone
- 6A5
- Applications
- ELISA, WB, IF, IP
- Conjugate
- Uconjugated
- Related Disease
- Tumor
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence, Immunoprecipitation.
WB:1:500-1:5000, IF:1:20-1:200, IP:1:200-1:1000
Target
- Alternative Names
- P59; ILK-1; ILK-2; p59ILK; HEL-S-28; integrin-linked protein kinase; 59 kDa serine/threonine-protein kinase; beta-integrin-linked kinase; epididymis secretory protein Li 28; integrin-linked kinase-2
- Gene ID
- 3611
- UniProt ID
- Q13418
Related Resources
Endometrial Cancer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "ILK"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2028MZ | Recombinant Mouse Anti-Human ILK Antibody | WB | Mouse antibody |
| MRO-0857-CN | Rabbit Anti-ILK Recombinant Antibody (clone CBACN-317) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-XY879 | Mouse Anti-ILK Recombinant Antibody (clone 3A9) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS3-WK931 | Rabbit Anti-ILK Recombinant Antibody (VS3-WK931) | WB, ICC, IHC, IP, FC | Rabbit IgG |
| VS3-FY755 | Recombinant Rabbit Anti-ILK Antibody (clone R01-7F8) | WB, IP | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1821 | Rabbit Anti-ILK Recombinant Antibody (clone DS1821AB) | WB, FC, ICC, IF | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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