Rabbit Anti-IRAK4 Recombinant Antibody (clone 10H4)
CAT#: ZG-0770J
This product is a rabbit antibody that recognizes IRAK4.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-IRAK4 Antibody (ZG-0770J) in WB
Western Blot
Positive WB detected in: THP-1 whole cell lysate, U251 whole cell lysate
All lanes: IRAK4 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52, 38 kDa
Observed band size: 55 kDa
FC
Figure 2 Rabbit Anti-IRAK4 Antibody (ZG-0770J) in FC
Overlay histogram showing Jurkat cells stained with this product (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.
❮
❯
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & microtubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16685/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli, cytosol & microtubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22077/621_E10_5_selected.jpg
Normal Tissue
Figure 3 IHC staining of human colon
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells and smooth muscle cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16685/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human breast
Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22077/ihc_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
nuclear Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22077/49420_B_8_5.jpg
Normal Tissue
Figure 6 Pancreas
Exocrine glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22077/49420_A_1_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16685/37917_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22077/49420_A_6_6.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (U-937)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000198001-IRAK4
❮
❯
Specifications
- Immunogen
- A synthesized peptide derived from human IRAK4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human IRAK4
- Species Reactivity
- Human
- Clone
- 10H4
- Applications
- ELISA, WB, FC
- Conjugate
- Uconjugated
- Related Disease
- Recurrent invasive pneumococcal disease
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry.
WB:1:500-1:5000, FC:1:20-1:200
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for ZG-0770J. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Signaling Pathways
TLR Signaling Pathway
Related Diseases
Maturity Onset Diabetes of the Young
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "IRAK4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2079z | Mouse Anti-IRAK4 Recombinant Antibody (clone 27A3) | WB, ELISA, FC, IHC | Mouse IgG1 |
| MOB-0992CT | Recombinant Mouse anti-Human IRAK4 Monoclonal antibody (A8A8) | ELISA, IHC-P, WB | |
| VS3-FY761 | Recombinant Rabbit Anti-IRAK4 Antibody (clone R01-1B2) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| VS7-0425-WR149 | Mouse Anti-IRAK4 Monoclonal Antibody (VS7-0425-WR149) | WB, IHC-P, IF, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0587CL-S(P) | Mouse Anti-IRAK4 Recombinant Antibody; scFv Fragment (TAB-0587CL-S(P)) | ELISA | Mouse scFv |
| TAB-0587CL-F(E) | Mouse Anti-IRAK4 Recombinant Antibody; Fab Fragment (TAB-0587CL-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1852 | Hi-Affi™ Recombinant Rabbit Anti-IRAK4 Monoclonal Antibody (DS1852AB) | ICC, WB | IgG |
| MOR-4632 | Hi-Affi™ Recombinant Rabbit Anti-IRAK4 Monoclonal Antibody (TH145DS) | WB, IF, ICC, IHC-P, FC, ChIP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY284 | Mouse Anti-NHP IRAK4 Recombinant Antibody (clone 2H9) | WB, IHC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3582 | Anti-IRAK4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3583 | Anti-Human IRAK4 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, IF, IP, Neut, FuncS, ELISA, FC
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: Neut, ELISA, FuncS
Application: ELISA, IP, WB, IHC, IF, FuncS
Application: WB, IF, FuncS
Application: FC, IB, Block, Inhib, FuncS, ELISA, FACS, IP, IF
Application: ELISA
Application: ELISA, Activ, Block
Application: IF, ICC, WB, IHC-P, IP
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
