Rabbit Anti-IRAK4 Recombinant Antibody (clone 10H4) (CAT#: ZG-0770J)
This product is a rabbit antibody that recognizes IRAK4.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-IRAK4 Antibody (ZG-0770J) in WB
Western Blot
Positive WB detected in: THP-1 whole cell lysate, U251 whole cell lysate
All lanes: IRAK4 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52, 38 kDa
Observed band size: 55 kDa
FC
Figure 2 Rabbit Anti-IRAK4 Antibody (ZG-0770J) in FC
Overlay histogram showing Jurkat cells stained with this product (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.
Specifications
- Immunogen
- A synthesized peptide derived from human IRAK4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human IRAK4
- Species Reactivity
- Human
- Clone
- 10H4
- Applications
- ELISA, WB, FC
- Conjugate
- Uconjugated
- Related Disease
- Recurrent invasive pneumococcal disease
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry.
WB:1:500-1:5000, FC:1:20-1:200
Target
- Alternative Names
- IPD1; IMD67; REN64; IRAK-4; NY-REN-64; interleukin-1 receptor-associated kinase 4; renal carcinoma antigen NY-REN-64
- Gene ID
- 51135
- UniProt ID
- Q9NWZ3
Related Resources
TLR Signaling Pathway
Maturity Onset Diabetes of the Young
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "IRAK4"
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IgG Antibody Products
Mouse Antibody Products
Rabbit Monoclonal Antibody Products
Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2079z | Mouse Anti-IRAK4 Recombinant Antibody (clone 27A3) | WB, ELISA, FC, IHC | Mouse IgG1 |
| MOB-0992CT | Recombinant Mouse anti-Human IRAK4 Monoclonal antibody (A8A8) | ELISA, IHC-P, WB | |
| VS3-FY761 | Recombinant Rabbit Anti-IRAK4 Antibody (clone R01-1B2) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| VS7-0425-WR149 | Mouse Anti-IRAK4 Monoclonal Antibody (VS7-0425-WR149) | WB, IHC-P, IF, FC, ELISA | Mouse IgG |
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