Rabbit Anti-ISG15 Recombinant Antibody (clone R06-6K4) (CAT#: VS3-XY3398)
This antibody is a recombinant rabbit antibody targeting the ISG15 (ISG15 ubiquitin like modifier), which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein. It specifically reacts with human ISG15. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin and Immunofluorescence is 1/500-1/1000, 1/50-1/100 and 1/50-1/200, respectively.
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Normal Tissue
RNA Expression
Figure 1 IHC staining of human liver
(Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/ihc_selected.jpg
(Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/ihc_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_B_7_5.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_B_7_5.jpg
Figure 3 Colon
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_8_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_8_3.jpg
Figure 4 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_8_4.jpg
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_8_4.jpg
Figure 5 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_7_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_7_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_4_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_4_6.jpg
Figure 7 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_9_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4627/12806_A_9_8.jpg
Figure 8 RNA cell line category: Cell line enhanced (BJ hTERT+ SV40 Large T+, BJ hTERT+ SV40 Large T+ RasG12V, HeLa, SK-MEL-30)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187608-ISG15
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187608-ISG15
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ISG15
- Species Reactivity
- Human
- Clone
- R06-6K4
- Applications
- WB, IHC-P, ICC, IF
- Related Disease
- Immunodeficiency 38 With Basal Ganglia Calcification
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- G1P2; IP17; UCRP; IFI15; IMD38; hUCRP
- Gene ID
- 9636
- UniProt ID
- P05161
- Long Name
- ISG15 ubiquitin like modifier
- Cellular Localization
- Cytoplasm, Secreted
- Post Translation Modifications
- Ubiquitination at Lys35 and Lys77 (NX_P05161 [NX_P05161-1])
Modification sites at PhosphoSitePlus (P05161)
Glycosylation from GlyGen (P05161) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_005092.1
- Function
- Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein.
ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1007MZ | Recombinant Mouse Anti-Human ISG15 Antibody (clone 4F6) | ELISA | Mouse antibody |
| MOB-125CQ | Mouse Anti-Mouse ISG15 Antibody | ELISA, WB, IP, IF | Mouse IgG2a, κ |
| VS3-CJ400 | Mouse Anti-ISG15 Recombinant Antibody (VS3-CJ400) | WB | |
| VS4-WK253 | Rabbit Anti-ISG15 Recombinant Antibody (clone 16D8) | ELISA, IHC, FC | Rabbit IgG |
| VS3-FY767 | Recombinant Rabbit Anti-ISG15 Antibody (clone R08-1H5) | WB | Rabbit IgG |
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