CytoStream™ Rabbit Anti-ISL1 Recombinant Antibody (VS13-YC598) (CAT#: VS13-YC598)
The CytoStream™ Rabbit Anti-ISL1 Recombinant Antibody is specific for human, mouse and rat ISL1. ISL1 is part of the LIM/homeodomain family of transcription factors, crucial for insulin gene regulation and pancreatic cell lineage development, as well as motor neuron generation. Mutations in ISL1 are linked to maturity-onset diabetes of the young. The anti-ISL1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/1000).
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SH-SY5Y
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48613/1792_G11_2_selected.jpg
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48613/1792_G11_2_selected.jpg
Figure 2 IHC staining of human caudate
(Immunohistochemical staining of human caudate shows strong nuclear positivity in a subset of glial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57416/ihc_selected.jpg
(Immunohistochemical staining of human caudate shows strong nuclear positivity in a subset of glial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57416/ihc_selected.jpg
Figure 3 Cerebral cortex
(Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57416/132766_B_7_5.jpg
(Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57416/132766_B_7_5.jpg
Figure 4 Caudate
(Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57416/132766_B_7_7.jpg
(Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57416/132766_B_7_7.jpg
Figure 5 Liver
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57416/132766_A_7_4.jpg
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57416/132766_A_7_4.jpg
Figure 6 Kidney
(Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57416/132766_A_7_5.jpg
(Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57416/132766_A_7_5.jpg
Figure 7 Testis
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57416/132766_A_5_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57416/132766_A_5_6.jpg
Figure 8 RNA cell line category: Cell line enriched (SH-SY5Y)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000016082-ISL1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000016082-ISL1
Specifications
- Immunogen
- Recombinant human Islet 1, aa 262-205.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ISL1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, IHC-Fr, ICC, IF, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/2000
Immunofluorescence (IF-cell): 1/100
Immunohistochemistry-paraffin (IHC-P): 1/1000
Flow cytometry (FC): 1/1000
Immunohistochemistry-frozen (IHC-Fr): 1/500
Target
- Alternative Names
- ISL LIM Homeobox 1
- Gene ID
- 3670
- UniProt ID
- P61371
- Long Name
- ISL LIM Homeobox 1
- Cellular Localization
- Nucleus
- Protein Refseq
- NP_002193.2
- Function
- DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1. Binds to insulin gene enhancer sequences. Essential for heart development. Marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells as well, its function is required for these progenitors to contribute to the heart. Controls the expression of FGF and BMP growth factors in this cell population and is required for proliferation and survival of cells within pharyngeal foregut endoderm and adjacent splanchnic mesoderm as well as for migration of cardiac progenitors into the heart (By similarity).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "ISL1"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1009MZ | Recombinant Mouse Anti-Human ISL1 Antibody (clone 2C2) | ELISA, WB sELISA | Mouse antibody |
| ZG-0512J | Mouse Anti-ISL1 Recombinant Antibody (ZG-0512J) | WB, IF, ELISA | Mouse IgG |
| ZG-0772J | Rabbit Anti-ISL1 Recombinant Antibody (clone 4F6) | ELISA, WB | Rabbit IgG |
| VS7-0425-WR150 | Mouse Anti-ISL1 Monoclonal Antibody (VS7-0425-WR150) | WB, IHC-P, IF, ICC, ELISA | Mouse IgG |
| VS7-0425-WR151 | Mouse Anti-ISL1 Monoclonal Antibody (VS7-0425-WR151) | WB, IF, ICC, ELISA | Mouse IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS13-YC598. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
-1-1.png)
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
-1-1.png)
Application: ELISA, WB, Neut, Funcs, IHC, Inhib
Application: ELISA, FC, IF, WB
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, FC, Inhib
Application: Neut, IHC, Activ, FuncS, IF, ELISA
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
