Rabbit Anti-ITGAX Recombinant Antibody (clone R06-5A7)
CAT#: VS3-XY3401
This antibody is a recombinant rabbit antibody targeting the ITGAX (Integrin subunit alpha X), which mediates cell-cell interaction during inflammatory responses. It specifically reacts with human ITGAX. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation and Immunofluorescence is 1/500-1/1000, 1/50-1/100, 1/20 and 1/50-1/200, respectively.
Gene Expression
Normal Tissue
Figure 1 High expression in spleen
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4723/14046_B_7_4_selected.jpg
Normal Tissue
Figure 2 Low expression in cerebral cortex
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4723/14046_B_9_5_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4458/14042_B_7_5.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4458/14042_A_7_8.jpg
Normal Tissue
Figure 5 Spleen
Cells in red pulp Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in white pulp Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4458/14042_B_8_4.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enriched (HMC-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000140678-ITGAX
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ITGAX
- Species Reactivity
- Human
- Clone
- R06-5A7
- Applications
- WB, IHC-P, ICC, IF, IP
- Related Disease
- Hairy Cell Leukemia; Sporotrichosis
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
Target
- Alternative Names
- CD11C; SLEB6
- Gene ID
- 3687
- UniProt ID
- P20702
- Long Name
- Integrin subunit alpha X
- Cellular Localization
- Membrane
- Post Translation Modifications
- Glycosylation at Asn61, Asn89, Asn392, Asn697, Asn735, Asn899, Asn904, Asn939, and Asn1050 (NX_P20702 [NX_P20702-1])
Modification sites at PhosphoSitePlus (P20702)
Glycosylation from GlyConnect ITAX_HUMAN (1413)
Glycosylation from GlyGen (P20702) 8 sites, 2 N-linked glycans (3 sites)
- Protein Refseq
- XP_011544154.1; XP_011544156.1
- Function
- Integrin alpha-X/beta-2 is a receptor for fibrinogen.
It recognizes the sequence G-P-R in fibrinogen.
It mediates cell-cell interaction during inflammatory responses.
It is especially important in monocyte adhesion and chemotaxis.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ITGAX"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3769z | Mouse Anti-ITGAX Recombinant Antibody (clone 20F12) | WB, ELISA, FC | Mouse IgG1 |
| MRO-0271-CN | Recombinant Rabbit Anti-ITGAX Monoclonal Antibody (CBACN-105) | WB, IHC, IP | Rabbit IgG |
| VS3-XY921 | Mouse Anti-ITGAX Recombinant Antibody (clone 4ALM2/1.6.11) | FC, IF | Mouse IgG1 |
| VS3-XY924 | Mouse Anti-ITGAX Recombinant Antibody (clone 3G1) | WB, IP, IF, IHC | Mouse IgG1 |
| VS3-XY925 | Mouse Anti-ITGAX Recombinant Antibody (clone 428CT12.2.6.4) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1580CL | Hamster Anti-ITGAX Recombinant Antibody (TAB-1580CL) | FC | Hamster IgG1, κ |
| TAB-1580CL-S(P) | Hamster Anti-ITGAX Recombinant Antibody; scFv Fragment (TAB-1580CL-S(P)) | FC | Hamster scFv |
| TAB-1580CL-F(E) | Hamster Anti-ITGAX Recombinant Antibody; Fab Fragment (TAB-1580CL-F(E)) | FC | Hamster Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0297MZ | Chicken Anti-ITGAX Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1584CQ | Recombinant Mouse Anti-ITGAX Antibody (3.9) | FC, CyTOF, BL, IHC | IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1881 | Hi-Affi™ Recombinant Rabbit Anti-ITGAX Monoclonal Antibody (DS1881AB) | ICC, IHC-P, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0304-CN-S(P) | Hamster Anti-ITGAX Recombinant Antibody (clone N418); scFv Fragment | WB, ELISA, IHC | Hamster scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0304-CN-F(E) | Hamster Anti-ITGAX Recombinant Antibody (clone N418); Fab Fragment | WB, ELISA, IHC | Hamster Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY74 | Mouse Anti-NHP ITGAX Recombinant Antibody (clone BU15) | FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY151 | CytoStream™ Armenian Hamster Anti-ITGAX Recombinant Antibody (VS-0225-XY151) | FC | Armenian Hamster IgG1 |
| VS13-YC601 | CytoStream™ Hamster Anti-Itgax Recombinant Antibody (VS13-YC601) | FC | Armenian hamster IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC235 | Recombinant Anti-ITGAX Vesicular Antibody, EV Displayed (VS-0425-YC235) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3647 | Anti-ITGAX Immunohistochemistry Kit | IHC | |
| VS-0525-XY3648 | Anti-Mouse ITGAX Immunohistochemistry Kit | IHC | |
| VS-0525-XY3650 | Anti-Human ITGAX Immunohistochemistry Kit | IHC |
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