Mouse Anti-ITGB2 Monoclonal Antibody (VS7-0425-WR160) (CAT#: VS7-0425-WR160)

• Immunogen
• Recombinant human ITGB2 fragment expressed in E. Coli.

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human ITGB2

• Species Reactivity
• Human, Mouse

• Molecule Class
• Integrin

• Applications
• IF, ICC, ELISA

• Conjugate
• Unconjugated

• Related Disease
• Leukocyte adhesion deficiency (LAD).
  Increased susceptibility to infections.
  Involved in long term pulmonary complications after COVID-19 infection.

• Clonality
• Monoclonal

• Purity
• >95% as determined by SDS-PAGE and SEC-HPLC analysis

• Format
• Liquid

• Concentration
• 1 mg/mL

• Preservative
• 0.03% sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• Immunofluorescence: 1:200 - 1:1000
  Enzyme-linked Immunosorbent Assay: 1:10000

• Alternative Names
• LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1

• Gene ID
• 3689

• UniProt ID
• P05107

• Long Name
• Integrin Subunit Beta 2

• Cellular Localization
• Cell membrane, Membrane

• Post Translation Modifications
• Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters (PubMed:11700305).
  Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins (PubMed:11700305, PubMed:16301335).

• Protein Refseq
• NP_000202.3

• Function
• Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (PubMed:29100055).
  Integrins ITGAM/ITGB2 and ITGAX/ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX/ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM/ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin ITGAM/ITGB2 is also a receptor for factor X. Integrin ITGAD/ITGB2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity (PubMed:15356110).
  Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed:11812992, PubMed:28807980).
  Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (PubMed:18587400).
  Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590).
  In association with alpha subunit ITGAM/CD11b, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407).

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

• 2ND-042CQ
• Rabbit Anti-Mouse IgG (H&L) Antibody

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