Mouse Anti-ITGB3 Recombinant Antibody (clone SZ-21) (CAT#: HPAB-0030-YJ)

Figure 1 Western blot analysis of platelet lysates.

The platelet lysate (10 µg protein) was subjected to Western blot analysis using monoclonal antibodies against αIIb (SZ22) or β3 (SZ21) as a primary antibody and HRP‐conjugated antimouse IgG (H+L) as a secondary antibody. In parallel, monoclonal antibody against GPIb (WGA‐3) was used as a primary antibody of a positive control. F, father, M, mother, GT, Glanzmann's thrombasthenia (1st child); B, younger brother (2nd child).

Tanaka, S., Hayashi, T., Terada, C., Hori, Y., Han, K. S., Ahn, H. S., ... & Tani, Y. (2003). Glanzmann's thrombasthenia due to a point mutation within intron 10 results in aberrant splicing of the β3 gene. Journal of Thrombosis and Haemostasis,  1(11), 2427-2433.

Figure 2 Immunoprecipitation of αIIbβ3 by adding the specific αIIb antibody.

The lysates of transfected COS7 were immunoprecipated by adding the specific αIIb monoclonal antibody M‐148 (Santa Cruz Biotechnology, Inc.) and protein G Sepharose 4 Fast Flow (Pharmacia Biotech.). The proteins eluted by heating the protein G Sepharose 4 Fast Flow with non‐reducing loading buffer was separated by 7.5% SDS‐PAGE, and were blotted to the polyvinylidene fluoride membrane. The blotted membrane was stained with the specific β3 monoclonal antibody SZ‐21 (Beckman Coulter) and AP‐conjugated antimouse IgG (H+L). The bands were visualized by the addition of NBT‐BCIP.

Tanaka, S., Hayashi, T., Terada, C., Hori, Y., Han, K. S., Ahn, H. S., ... & Tani, Y. (2003). Glanzmann's thrombasthenia due to a point mutation within intron 10 results in aberrant splicing of the β3 gene. Journal of Thrombosis and Haemostasis, 1(11), 2427-2433.

Figure 3 Flow cytometry analysis of the expression of the mutant forms of αIIbβ3. 235x165mm (96 x 96 DPI)

Jallu, V., Dusseaux, M., Panzer, S., Torchet, M. F., Hezard, N., Goudemand, J., ... & Kaplan, C. (2010). αIIbβ3 integrin: new allelic variants in Glanzmann thrombasthenia, effects on ITGA2B and ITGB3 mRNA splicing, expression, and structure–function. Human mutation, 31(3), 237-246.