Mouse Anti-ITGB3 Recombinant Antibody (ZG-0126J)
CAT#: ZG-0126J
This product is a mouse antibody that recognizes ITGB3.
Tested Data
Gene Expression
IHC
Figure 1 Mouse Anti-ITGB3 Recombinant Antibody (ZG-0126J) in IHC
Immunohistochemical analysis of paraffin-embedded Spleen. 1. Antibody was diluted at 1:200 (4°C overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min).
IHC
Figure 2 Mouse Anti-ITGB3 Recombinant Antibody (ZG-0126J) in IHC
Immunohistochemical analysis of paraffin-embedded LIver. 1. Antibody was diluted at 1:200 (4°C overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min).
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27852/281_B9_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in megakaryocytes.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27852/ihc_selected.jpg
Normal Tissue
Figure 3 Bone marrow
Hematopoietic cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27852/64673_B_4_4.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (EFO-21, HEL, RPTEC TERT1, U-87 MG, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000259207-ITGB3
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Specifications
- Immunogen
- Synthesized peptide derived from human CD61
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human ITGB3
- Species Reactivity
- Human, Mouse
- Applications
- IHC-P, IF (paraffin section)
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunohistochemistry-Paraffin, Immunofluorescence (paraffin section).
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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