SmartAb™ Recombinant Anti-ITGB8 pH-dependent Antibody (VS-0825-YC230)
CAT#: VS-0825-YC230
The anti-ITGB8 pH-dependent antibody (VS-0825-YC230) targets human ITGB8 in a pH-dependent manner, with its affinity remaining high at neutral pH but weakening at a lower pH, such as within the acidic environment of endosomes. The anti-ITGB8 acid-switched antibody was engineered by incorporating histidine residues into its variable regions. The introduction of histidine substitutions within its Complementarity-Determining Regions (CDRs) enables pH-responsive antigen binding, which leads to enhanced ITGB8 clearance. The antibody operates in a cyclical manner, binding ITGB8 in the extracellular fluid and releasing it in acidified endosomes. Human ITGB8 is an integrin subunit that is a receptor for extracellular matrix proteins and a key activator of latent TGF-β. It plays a role in nervous system development and is often overexpressed in glioblastoma, making it a potential therapeutic target.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27796/231_A10_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27797/165151_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27797/165151_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27797/165151_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27797/165151_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27797/165151_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (AF22, EFO-21, RPTEC TERT1, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105855-ITGB8
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human ITGB8
- Species Reactivity
- Human
- Applications
- ELISA
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE and SEC-HPLC
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- For short-term storage, keep it at 4°C for up to two weeks. For long-term storage, divide the solution into aliquots and freeze them at -20°C or below.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "ITGB8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2027MZ | Recombinant Mouse Anti-Human ITGB8 Antibody | WB | Mouse antibody |
| HPAB-0400-WJ | Mouse Anti-ITGB8 Recombinant Antibody (HPAB-0400-WJ) | ELISA | Mouse IgG |
| HPAB-0401-WJ | Mouse Anti-ITGB8 Recombinant Antibody (HPAB-0401-WJ) | ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0303MZ | Chicken Anti-ITGB8 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0400-WJ-S(P) | Mouse Anti-ITGB8 Recombinant Antibody; scFv Fragment (HPAB-0400-WJ-S(P)) | ELISA | Mouse scFv |
| HPAB-0401-WJ-S(P) | Mouse Anti-ITGB8 Recombinant Antibody; scFv Fragment (HPAB-0401-WJ-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0400-WJ-F(E) | Mouse Anti-ITGB8 Recombinant Antibody; Fab Fragment (HPAB-0400-WJ-F(E)) | ELISA | Mouse Fab |
| HPAB-0401-WJ-F(E) | Mouse Anti-ITGB8 Recombinant Antibody; Fab Fragment (HPAB-0401-WJ-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0917 | PE-A*02:01/Human ITGB8 (ALMEQQHYV) MHC Tetramer | FCM, IHC | |
| MHC-CN0918 | APC-A*02:01/Human ITGB8 (ALMEQQHYV) MHC Tetramer | FCM, IHC | |
| MHC-CN0919 | FITC-A*02:01/Human ITGB8 (ALMEQQHYV) MHC Tetramer | FCM, IHC | |
| MHC-CN0920 | A*02:01/Human ITGB8 (ALMEQQHYV) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC569 | Recombinant Anti-ITGB8 Vesicular Antibody, EV Displayed (VS-0425-YC569) | ELISA, FC, Cell-uptake |
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