Recombinant Mouse Anti-J chains Antibody (JC4) (CAT#: FAMAB-0049-CN)

Figure 1 Isolation of J chain from polymeric IgM.

A, Whole serum was subjected to SDS-PAGE under nonreducing conditions to separate polymericand monomeric IgM, which were then excised from the gel and subjected to SDS-PAGE under reducing conditions. A doublet corresponding to J chainis present in the polymeric, but not the monomeric IgM. Band C, Nurse shark serum was immunoprecipitated with mouse mAbs JC4 (preferentiallyprecipitates polymeric IgM) and GA15 (preferentially precipitates monomeric IgM). Unreduced immunoprecipitates were applied to a 5% polyacrylamidegel (B), and reduced immunoprecipitates to a 12% gel (C). A J chain doublet is present in the reduced IgM precipitated with JC4, but not in the reducedIgM precipitated with GA15. H, L, and J chains from mouse and shark Ig are indicated. The band that is present between the shark L chains and J chainsin the JC4 immunoprecipitation is from the JC4 Ab preparation. In the no Ab control, serum was combined with protein G beads only. Bands in the gelswere visualized by staining with Coomassie blue.

Hohman, V. S., Stewart, S. E., Rumfelt, L. L., Greenberg, A. S., Avila, D. W., Flajnik, M. F., & Steiner, L. A. (2003). J chain in the nurse shark: implications for function in a lower vertebrate. The Journal of Immunology, 170(12), 6016-6023.