Mouse Anti-JAK1 Recombinant Antibody (clone 3F12) (CAT#: VS3-WK1613)
This antibody is a mouse recombinant antibody against human JAK1, which plays a crucial role in effecting the expression of genes that mediate inflammation, epithelial remodeling, and metastatic cancer progression. The clone 3F12 can be used in WB analysis at a dilution of 1/1000-1/2000 and IHC analysis at a dilution of 1/50-1/200.
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in non-germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13088/33500_A_6_8_selected.jpg
(Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in non-germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13088/33500_A_6_8_selected.jpg
Figure 2 Cerebral cortex
(Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13088/33500_B_8_5.jpg
(Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13088/33500_B_8_5.jpg
Figure 3 Colon
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13088/33500_A_8_3.jpg
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13088/33500_A_8_3.jpg
Figure 4 Liver
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13088/33500_A_7_4.jpg
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13088/33500_A_7_4.jpg
Figure 5 Kidney
(Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13088/33500_A_8_5.jpg
(Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13088/33500_A_8_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13088/33500_A_4_6.jpg
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13088/33500_A_4_6.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13088/33500_A_9_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13088/33500_A_9_8.jpg
Figure 8 RNA cell line category: Cell line enhanced (ASC diff)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162434-JAK1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162434-JAK1
Specifications
- Immunogen
- Synthesized peptide derived from human JAK1
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human JAK1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 3F12
- Applications
- IHC-P, WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 0.5% BSA, 50% Glycerol
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- IHC-P: 1/50-1/200
WB: 1/1000-1/2000
Target
- Alternative Names
- JTK3; AIIDE; JAK1A; JAK1B
- Gene ID
- 3716
- UniProt ID
- P23458
- Long Name
- Janus kinase 1
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
- Cellular Localization
- Membrane
- Post Translation Modifications
- Phosphorylated by TYK2 on tyrosine residues in response to type-I interferon signaling, leading to its activation.
Phosphorylated on tyrosine residues in response to interferon gamma signaling.
Dephosphorylation of Tyr-1034 and Tyr-1035 by PTPN2 negatively regulates cytokine-mediated signaling.
- Protein Refseq
- NP_001307852.1
- Function
- Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway.
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
Pancreatic Cancer
Maturity Onset Diabetes of the Young
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC206 | PE-H-2Kd/Human JAK1 (SYFPEITHI) MHC Tetramer | FCM | |
| MHC-LC207 | APC-H-2Kd/Human JAK1 (SYFPEITHI) MHC Tetramer | FCM |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1897 | Hi-Affi™ Recombinant Rabbit Anti-JAK1 Monoclonal Antibody (DS1897AB) | WB | IgG |
| MOR-4690 | Hi-Affi™ Recombinant Rabbit Anti-JAK1 Monoclonal Antibody (TH204DS) | WB, IF, ICC, ChIP | IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0879-CN | Recombinant Rabbit Anti-JAK1 Monoclonal Antibody (JM75-03) | WB, IHC, FC | Rabbit IgG |
| ZG-0516J | Mouse Anti-JAK1 Recombinant Antibody (ZG-0516J) | WB | Mouse IgG |
| ZG-0517J | Mouse Anti-JAK1 Recombinant Antibody (ZG-0517J) | WB, ICC | Mouse IgG |
| VS3-QX613 | Mouse Anti-JAK1 Recombinant Antibody (clone 8D8-2B5-5E3) | IHC | Mouse IgG1 |
| VS3-QX614 | Mouse Anti-JAK1 Recombinant Antibody (clone 8B8-E7-G3) | WB, IF, IP | Mouse IgG2a |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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