Mouse Anti-JAK1 Recombinant Antibody (clone 3F12)
CAT#: VS3-WK1613
This antibody is a mouse recombinant antibody against human JAK1, which plays a crucial role in effecting the expression of genes that mediate inflammation, epithelial remodeling, and metastatic cancer progression. The clone 3F12 can be used in WB analysis at a dilution of 1/1000-1/2000 and IHC analysis at a dilution of 1/50-1/200.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in non-germinal center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13088/33500_A_6_8_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13088/33500_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13088/33500_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13088/33500_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13088/33500_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13088/33500_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13088/33500_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (ASC diff)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162434-JAK1
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Specifications
- Immunogen
- Synthesized peptide derived from human JAK1
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human JAK1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 3F12
- Applications
- IHC-P, WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 0.5% BSA, 50% Glycerol
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- IHC-P: 1/50-1/200
WB: 1/1000-1/2000
Target
- Alternative Names
- JTK3; AIIDE; JAK1A; JAK1B
- Gene ID
- 3716
- UniProt ID
- P23458
- Long Name
- Janus kinase 1
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
- Cellular Localization
- Membrane
- Post Translation Modifications
- Phosphorylated by TYK2 on tyrosine residues in response to type-I interferon signaling, leading to its activation.
Phosphorylated on tyrosine residues in response to interferon gamma signaling.
Dephosphorylation of Tyr-1034 and Tyr-1035 by PTPN2 negatively regulates cytokine-mediated signaling.
- Protein Refseq
- NP_001307852.1
- Function
- Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
Isotype Control
- CAT
- Product Name
See other products for "JAK1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2481z | Mouse Anti-JAK1 Recombinant Antibody (clone 5B5) | WB, ICC, IF, IHC, IP | Mouse IgG1 |
| MRO-0879-CN | Recombinant Rabbit Anti-JAK1 Monoclonal Antibody (JM75-03) | WB, IHC, FC | Rabbit IgG |
| VS3-QX613 | Mouse Anti-JAK1 Recombinant Antibody (clone 8D8-2B5-5E3) | IHC | Mouse IgG1 |
| VS3-QX614 | Mouse Anti-JAK1 Recombinant Antibody (clone 8B8-E7-G3) | WB, IF, IP | Mouse IgG2a |
| VS3-QX615 | Mouse Anti-JAK1 Recombinant Antibody (clone 7B1-B6-G7) | WB, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC206 | PE-H-2Kd/Human JAK1 (SYFPEITHI) MHC Tetramer | FCM | |
| MHC-LC207 | APC-H-2Kd/Human JAK1 (SYFPEITHI) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1897 | Hi-Affi™ Recombinant Rabbit Anti-JAK1 Monoclonal Antibody (DS1897AB) | WB | IgG |
| MOR-4690 | Hi-Affi™ Recombinant Rabbit Anti-JAK1 Monoclonal Antibody (TH204DS) | WB, IF, ICC, ChIP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1162 | Anti-JAK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3686 | Anti-Mouse JAK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3685 | Anti-Human JAK1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC608 | CytoStream™ Rabbit Anti-JAK1 Recombinant Antibody (VS13-YC608) | WB, IHC-P, FC | Rabbit IgG |
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