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    Mouse Anti-JAK2 Recombinant Antibody (clone 5C12C9) (CAT#: VS7-0425-WR167)

    This product is a mouse IgG1 antibody that binds to human JAK2. The antibody 5C12C9 recognizes specific amino acid domain of human JAK2 (AA: 745-955). It can be used for IHC and FC assays.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & focal adhesion sites.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/43870/1338_A2_1_selected.jpg
    Normal Tissue
    Figure 2 IHC staining of human bone marrow
    Figure 2 IHC staining of human bone marrow
    (Immunohistochemical staining of human bone marrow shows moderate cytoplasmic and nuclear positivity in hematopoietic cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/40820/ihc_selected.jpg
    Figure 3 IHC staining of human lymph node
    Figure 3 IHC staining of human lymph node
    (Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in lymphoid tissue.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/13089/ihc_selected.jpg
    Figure 4 Cerebral cortex
    Figure 4 Cerebral cortex
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/13089/29841_B_7_5.jpg
    Figure 5 Colon
    Figure 5 Colon
    (Endothelial cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/13089/29841_A_9_3.jpg
    Figure 6 Liver
    Figure 6 Liver
    (Hepatocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/13089/29841_A_8_4.jpg
    Figure 7 Kidney
    Figure 7 Kidney
    (Cells in glomeruli
    Staining: High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Cells in tubules
    Staining: High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/13089/29841_A_9_5.jpg
    Figure 8 Testis
    Figure 8 Testis
    (Cells in seminiferous ducts
    Staining: Medium
    Intensity: Strong
    Quantity: <25%
    Location: Cytoplasmic/membranous
    Leydig cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/13089/29841_A_4_6.jpg
    RNA Expression
    Figure 9 RNA cell line category: Group enriched (ASC diff, HDLM-2, HEL)
    Figure 9 RNA cell line category: Group enriched (ASC diff, HDLM-2, HEL)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000096968-JAK2

    Specifications

    • Immunogen
    • Recombinant human JAK2 (AA: 745-955) fragment expressed in E. Coli.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG1
    • Specificity
    • Human JAK2
    • Species Reactivity
    • Human
    • Clone
    • 5C12C9
    • Molecule Class
    • Kinase
    • Applications
    • IHC, FC
    • Conjugate
    • Unconjugated

    Product Property

    • Clonality
    • Monoclonal
    • Purity
    • >95% as determined by SDS-PAGE and SEC-HPLC analysis
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS
    • Preservative
    • 0.05% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • Western Blot: 1:500 - 1:2000
      Immunohistochemistry-Paraffin: 1:200 - 1:1000
      Flow Cytometry: 1:200 - 1:400
      Enzyme-linked Immunosorbent Assay: 1:10000

    Target

    • Alternative Names
    • JAK2; Tyrosine-Protein Kinase JAK2; JAK-2; Janus Kinase 2 (A Protein Tyrosine Kinase); JTK10; EC 2.7.10; Janus Kinase 2; THCYT3; EC 2.7.10.2
    • Long Name
    • Janus kinase 2
    • Cellular Localization
    • Cytoplasm; Membrane; Nucleus
    • Post Translation Modifications
    • Phosphoprotein; Ubl conjugation
    • Function
    • Chromatin regulator; Kinase; Transferase; Tyrosine-protein kinase

    Recommended Products

    Isotype Control

    Secondary Antibody

    Reconstitution Buffer

    Recommended Dilution Buffer

    Related Resources

    • Related Signaling Pathways
    • Related Diseases

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "JAK2"

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    MOB-2300CT Recombinant Mouse anti-Human JAK2 Monoclonal antibody (Z377) WB
    MOB-0064F Mouse Anti-JAK2 Recombinant Antibody (clone 3F10) IHC-P, IF Mouse IgG
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