Rabbit Anti-JUNB Recombinant Antibody (clone 5H4) (CAT#: ZG-0042U)
The purified Rabbit Anti-JUNB Recombinant Antibody (clone 5H4) displayed high specificity against the JUNB protein of human.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
IHC
Figure 1 Rabbit Anti-JUNB Antibody (ZG-0042U) in IHC
IHC image of ZG-0042U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IF
Figure 2 Rabbit Anti-JUNB Antibody (ZG-0042U) in IF
Immunofluorescence staining of MCF7 Cells with ZG-0042U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human JunB
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human JUNB
- Species Reactivity
- Human
- Clone
- 5H4
- Applications
- ELISA, IHC, IF
- Conjugate
- Unconjugated
- Related Disease
- Anaplastic Large Cell Lymphoma and Primary Cutaneous T-Cell Lymphoma
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunofluorescence. Specific conditions should be optimized by the end user.
IHC:1:50-1:200, IF:1:20-1:200
Target
Related Resources
TNF Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "JUNB"
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IgG Antibody Products
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1430MZ | Recombinant Mouse Anti-Human JUNB Antibody (clone 305D5b) | WB | Mouse antibody |
| MRO-0887-CN | Recombinant Rabbit Anti-JUNB Monoclonal Antibody (CBACN-333) | WB, IF, IP | Rabbit IgG |
| VS3-CJ710 | Rabbit Anti-JUNB Recombinant Antibody (VS3-CJ710) | WB, ICC, IF, IP | Rabbit IgG |
| VS3-FY787 | Recombinant Rabbit Anti-JUNB Antibody (clone R01-9B7) | WB, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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