Mouse Anti-KDM1A Monoclonal Antibody (clone 1B2E5) (CAT#: VS7-0425-WR320)
This product is a mouse IgG1 antibody that binds to human KDM1A. The antibody 1B2E5 can be used for WB and IHC assays. It cross reacts with mouse and monkey.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53660/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53660/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/if_selected.jpg
Figure 3 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/15747_A_7_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/15747_A_7_4.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/15747_A_8_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/15747_A_8_5.jpg
Figure 5 Testis
(Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/15747_A_4_6.jpg
(Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/15747_A_4_6.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000004487-KDM1A
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000004487-KDM1A
Specifications
- Immunogen
- Recombinant human KDM1A fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human KDM1A
- Species Reactivity
- Human, Mouse, Monkey
- Clone
- 1B2E5
- Molecule Class
- Demethylase
- Applications
- WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Cleft palate, psychomotor retardation, and distinctive facial features (CPRF)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- KDM1A; KDM1; BHC110; Flavin-Containing Amine Oxidase Domain-Containing Protein 2; Lysine (K)-Specific Demethylase 1A; Amine Oxidase (Flavin Containing) Domain 2; Lysine-Specific Histone Demethylase 1A; Lysine (K)-Specific Demethylase 1; KIAA0601; LSD1; BR
- Gene ID
- 23028
- UniProt ID
- O60341
- Long Name
- Lysine demethylase 1A
- Cellular Localization
- Chromosome; Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001009999.1
- Function
- Chromatin regulator; Developmental protein; Oxidoreductase; Repressor
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4025z | Mouse Anti-KDM1A Recombinant Antibody (clone 24H8) | WB, ELISA, FC, ICC, IHC | Mouse IgG1 |
| MOB-2167MZ | Recombinant Mouse Anti-Human KDM1A Antibody | ELISA, ELISA, ICC/IF, IP, WB | Mouse antibody |
| MRO-2018-CN | Rabbit Anti-KDM1A Polyclonal Antibody (MRO-2018-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-QX634 | Mouse Anti-KDM1A Recombinant Antibody (clone 1E5-H2) | WB, ICC, IP | Mouse IgG1 |
| VS3-CJ10 | Mouse Anti-KDM1A Recombinant Antibody (VS3-CJ10) | WB, ELISA | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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