Mouse Anti-KDM1A Recombinant Antibody (clone 5B2D5)
CAT#: VS7-0425-WR190
This product is a mouse IgG1 antibody that binds to human KDM1A. The antibody 5B2D5 recognizes specific amino acid domain of human KDM1A (AA: 55-263). It can be used for WB, ICC, and FC assays. It cross reacts with monkey.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53660/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/if_selected.jpg
Normal Tissue
Figure 3 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/15747_A_7_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/15747_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/15747_A_4_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000004487-KDM1A
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Specifications
- Immunogen
- Recombinant human KDM1A (AA: 55-263) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human KDM1A
- Species Reactivity
- Human
- Clone
- 5B2D5
- Molecule Class
- Demethylase
- Applications
- WB, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Cleft palate, psychomotor retardation, and distinctive facial features (CPRF)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- KDM1A; KDM1; BHC110; Flavin-Containing Amine Oxidase Domain-Containing Protein 2; Lysine (K)-Specific Demethylase 1A; Amine Oxidase (Flavin Containing) Domain 2; Lysine-Specific Histone Demethylase 1A; Lysine (K)-Specific Demethylase 1; KIAA0601; LSD1; BR
- Gene ID
- 23028
- UniProt ID
- O60341
- Long Name
- Lysine demethylase 1A
- Cellular Localization
- Chromosome; Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001009999.1
- Function
- Chromatin regulator; Developmental protein; Oxidoreductase; Repressor
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4025z | Mouse Anti-KDM1A Recombinant Antibody (clone 24H8) | WB, ELISA, FC, ICC, IHC | Mouse IgG1 |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1924 | Hi-Affi™ Recombinant Rabbit Anti-KDM1A Monoclonal Antibody (DS1924AB) | WB, IHC, ICC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3746 | Anti-KDM1A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3747 | Anti-Mouse KDM1A Immunohistochemistry Kit | IHC |
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