Rabbit Anti-KDM1A Recombinant Antibody (clone R06-9E8)
CAT#: VS3-XY3404
It is a recombinant rabbit antibody that recognizes KDM1A (Lysine demethylase 1A), which acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. The antibody recognizes and binds to human, mouse and rat KDM1A. This antibody has been used in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53660/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/if_selected.jpg
Normal Tissue
Figure 3 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/15747_A_7_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/15747_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/15747_A_4_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000004487-KDM1A
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat KDM1A
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-9E8
- Applications
- WB, IHC-P, ICC, IF, IP
- Related Disease
- Cleft Palate; Psychomotor Retardation; Distinctive Facial Features; Neuroblastoma
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
Target
- Alternative Names
- AOF2; CPRF; KDM1; LSD1; BHC110
- Gene ID
- 23028
- UniProt ID
- O60341
- Long Name
- Lysine demethylase 1A
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Polyubiquitinated by JADE2; which leads to its proteasomal degradation (PubMed:25018020).
Deubiquitinated by USP38; preventing it from degradation by the 26S proteasome (PubMed:30497519).
(O60341-KDM1A_HUMAN)
Ubiquitination at Lys280, Lys322, Lys347, Lys355, Lys374, Lys492, Lys503, Lys647, Lys732, and Lys744 (NX_O60341 [NX_O60341-1])
Modification sites at PhosphoSitePlus (O60341)
Glycosylation from GlyGen (O60341) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_006710536.1
- Function
- Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context (PubMed:15620353, 15811342, 16140033, 16079794, 16079795, 16223729).
Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed (PubMed:15620353, 15811342, 16079794, 21300290).
Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation.
Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me (PubMed:15620353, 20389281, 21300290, 23721412).
May play a role in the repression of neuronal genes.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
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- Product Name
Recommended Dilution Buffer
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Secondary Antibody
- CAT
- Product Name
See other products for "KDM1A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1924 | Hi-Affi™ Recombinant Rabbit Anti-KDM1A Monoclonal Antibody (DS1924AB) | WB, IHC, ICC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2018-CN | Rabbit Anti-KDM1A Polyclonal Antibody (MRO-2018-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ10 | Mouse Anti-KDM1A Recombinant Antibody (VS3-CJ10) | WB, ELISA | Mouse IgG |
| VS7-0425-WR188 | Mouse Anti-KDM1A Recombinant Antibody (clone 1H2F8) | WB, IHC, ICC, FC | Mouse IgG1 |
| VS7-0425-WR189 | Mouse Anti-KDM1A Recombinant Antibody (clone 3F9D2) | WB, IHC, ICC, FC | Mouse IgG1 |
| VS7-0425-WR190 | Mouse Anti-KDM1A Recombinant Antibody (clone 5B2D5) | WB, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3746 | Anti-KDM1A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3747 | Anti-Mouse KDM1A Immunohistochemistry Kit | IHC |
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